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The gene expression profiles of WT and NML-KO livers

ABSTRACT: To examine whether energy starvation caused by the increase in rRNA transcription affects liver metabolism, we compared the gene expression profiles of WT and NML-KO livers using Affymetrix microarray technology. We analyzed 5 livers of WT mice and 5 livers of NML-KO mice.

ORGANISM(S): Mus musculus

SUBMITTER: Shohei Oie

PROVIDER: E-GEOD-48191 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hepatic rRNA transcription regulates high-fat-diet-induced obesity.

Oie Shohei S Matsuzaki Kazuya K Yokoyama Wataru W Tokunaga Shinji S Waku Tsuyoshi T Han Song-Iee SI Iwasaki Naoya N Mikogai Aya A Yasuzawa-Tanaka Kayoko K Kishimoto Hiroyuki H Hiyoshi Hiromi H Nakajima Yuka Y Araki Toshiyuki T Kimura Keiji K Yanagisawa Junn J Murayama Akiko A

Cell reports 20140418 3

Ribosome biosynthesis is a major intracellular energy-consuming process. We previously identified a nucleolar factor, nucleomethylin (NML), which regulates intracellular energy consumption by limiting rRNA transcription. Here, we show that, in livers of obese mice, the recruitment of NML to rRNA gene loci is increased to repress rRNA transcription. To clarify the relationship between obesity and rRNA transcription, we generated NML-null (NML-KO) mice. NML-KO mice show elevated rRNA level, reduce ...[more]

PMID: 24746822

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