Unknown,Transcriptomics,Genomics,Proteomics

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A miR-155-ruled microRNA hierarchy in dendritic cell maturation and macrophage activation

ABSTRACT: MicroRNAs (miRNAs) are small RNAs that play important regulatory roles in many cellular pathways. MiRNAs associate with members of the Argonaute (Ago) protein family and bind to partially complementary sequences on mRNAs and induce translational repression or mRNA decay. MiRNA expression can be controlled by transcription factors and can therefore be cell type- or tissue-specific. Here we have analyzed miRNA expression profiles in murine monocyte-derived dendritic cells (DCs) and macrophages upon stimulation with LPS, LDL, eLDL and oxLDL to identify not only stimuli-specific miRNA, but also to identify a hierarchical miRNA system involving miR-155. For this, miR-155 knockout dendritic cells and macrophages were also sequenced using the same stimuli. Sequencing of murine monocyte-derived dendritic cells and macrophages (each wild type and miR-155 knock out cells) matured and stimulated, respectively, by LPS, oxLDL, eLDL or LDL.

ORGANISM(S): Mus musculus

SUBMITTER: Anne Dueck

PROVIDER: E-GEOD-48404 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation.

Dueck Anne A Eichner Alexander A Sixt Michael M Meister Gunter G

FEBS letters 20140118 4

MicroRNAs (miRNAs) are small RNAs that play important regulatory roles in many cellular pathways. MiRNAs associate with members of the Argonaute protein family and bind to partially complementary sequences on mRNAs and induce translational repression or mRNA decay. Using deep sequencing and Northern blotting, we characterized miRNA expression in wild type and miR-155-deficient dendritic cells (DCs) and macrophages. Analysis of different stimuli (LPS, LDL, eLDL, oxLDL) reveals a direct influence ...[more]

PMID: 24444604

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A miR-155-ruled microRNA hierarchy in dendritic cell maturation and macrophage activation

Publications

A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation.

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