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The affect of specific ablation of Runx3 from Esam splenic dendritic cells

ABSTRACT: Esam/CD4+ dendritic cells are part of the innate immunity essential for priming and activating of CD4+ T cells To identify Runx3 responsive genes Esam dendritic cells were freshly sorted from macs enriched splenic DCs taken from 6 weeks old mice. Four samples from four mice were sorted and analyzed where in each littermates pair consisted of a control and Runx3 conditional KO. Mice lacking Runx3 specifically in the DC compartment were produced by crossing Runx3fl/fl mice onto CD11c-Cre mice. This mating scheme generated Runx3fl/fl/CD11c:Cre (CD11c-DC-Runx3Δ) mice.

ORGANISM(S): Mus musculus

SUBMITTER: Yoram Groner

PROVIDER: E-GEOD-48590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional reprogramming of CD11b+Esam(hi) dendritic cell identity and function by loss of Runx3.

Dicken Joseph J Mildner Alexander A Leshkowitz Dena D Touw Ivo P IP Hantisteanu Shay S Jung Steffen S Groner Yoram Y

PloS one 20131015 10

Classical dendritic cells (cDC) are specialized antigen-presenting cells mediating immunity and tolerance. cDC cell-lineage decisions are largely controlled by transcriptional factor regulatory cascades. Using an in vivo cell-specific targeting of Runx3 at various stages of DC lineage development we show that Runx3 is required for cell-identity, homeostasis and function of splenic Esam(hi) DC. Ablation of Runx3 in DC progenitors led to a substantial decrease in splenic CD4(+)/CD11b(+) DC. Combin ...[more]

PMID: 24204843

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Runx3 function in CD4+ splenic dendritic cells

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2014-06-22 | E-GEOD-48589 | biostudies-arrayexpress

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2014-01-01 | E-GEOD-52857 | biostudies-arrayexpress

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2011-10-31 | E-GEOD-31551 | biostudies-arrayexpress

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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2010-03-24 | E-GEOD-17322 | biostudies-arrayexpress

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