Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Translational activation of developmental mRNAs during neonatal mouse testis development


ABSTRACT: The sequence of gene regulatory events that drive neonatal germ cell development in the mammalian testis is not yet clear. We assessed changes in mRNA utilization in the neonatal testis at 1 and 4 dpp, times when the testis contains quiescent gonocytes (1 dpp) and proliferating spermatogonia (4 dpp). There are not thought to be major changes in the nature or number of somatic cells over that interval. We used microarrays to detail the global expression levels of mRNA distribution between non-translating mRNAs and efficiently translating mRNAs during testis development at 1 dpp and 4 dpp Extracts of mouse testes from 1 dpp and 4 dpp CD-1 mice were fractionated over a sucrose gradient in triplicate. Gradients were fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. The position of the 80S peak defined the boundary between translating and non-translating mRNAs. Pooled non-polysomal (non-translating; fractions 1-3) and heavy polysomal (efficiently translating, fractions 7-13) RNAs were isolated from sucrose gradient fractions for hybridization on Affymetrix 420 2.0 microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Christopher Geyer 

PROVIDER: E-GEOD-49283 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Translational activation of developmental messenger RNAs during neonatal mouse testis development.

Chappell Vesna A VA   Busada Jonathan T JT   Keiper Brett D BD   Geyer Christopher B CB  

Biology of reproduction 20130919 3


The basic tenets of germ cell development are conserved among metazoans. Following lineage commitment in the embryo, germ cells proliferate, transition into meiosis, and then differentiate into gametes capable of fertilization. In lower organisms such as Drosophila and C. elegans, germline stem cells make the decision to proliferate or enter meiosis based in large part on the regulated expression of genes by translational control. This study undertakes a direct characterization of mRNAs that exp  ...[more]

Similar Datasets

2012-06-20 | E-GEOD-38807 | biostudies-arrayexpress
2015-04-29 | E-MTAB-3537 | biostudies-arrayexpress
2014-06-12 | E-GEOD-52799 | biostudies-arrayexpress
2013-07-27 | GSE49283 | GEO
2007-11-23 | E-GEOD-4711 | biostudies-arrayexpress
2013-11-01 | E-GEOD-43296 | biostudies-arrayexpress
2013-02-17 | E-GEOD-41787 | biostudies-arrayexpress
2014-04-17 | E-GEOD-50650 | biostudies-arrayexpress
2010-07-07 | E-DORD-69 | biostudies-arrayexpress
2020-07-01 | E-MTAB-4016 | biostudies-arrayexpress