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ChIP-seq for PRC2 components and H3K27me3 in E14 mouse embryonic stem cells

ABSTRACT: This experiment sought to determine the chromatin structure and PRC2 occupancy at the promoters of all genes in mouse ESCs ChIP-seq for EZH2, SUZ12, and H3K27me3 were performed on WT E14 cells in 2 biological replicates each. In addition H3K27me3 ChIP-seq was also performed on a clone of E14 cells expressing a tagged version of EZH2, which for the purpose of this study was used as replicate #3.

ORGANISM(S): Mus musculus

SUBMITTER: Roberto Bonasio

PROVIDER: E-GEOD-49431 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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PRC2 binds active promoters and contacts nascent RNAs in embryonic stem cells.

Kaneko Syuzo S Son Jinsook J Shen Steven S SS Reinberg Danny D Bonasio Roberto R

Nature structural & molecular biology 20131020 11

EZH2 is the catalytic subunit of PRC2, a central epigenetic repressor essential for development processes in vivo and for the differentiation of embryonic stem cells (ESCs) in vitro. The biochemical function of PRC2 in depositing repressive H3K27me3 marks is well understood, but how it is regulated and directed to specific genes before and during differentiation remains unknown. Here, we report that PRC2 binds at low levels to a majority of promoters in mouse ESCs, including many that are active ...[more]

PMID: 24141703

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