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RNA seq in two lines of E14 mouse embryonic stem cells

ABSTRACT: This experiment was designed to obtain the polyA+ transcriptome in E14 ESCs PolyA+ RNA was extracted and purified from two separate clones of E14, which were treated as biological replicate

ORGANISM(S): Mus musculus

SUBMITTER: Roberto Bonasio

PROVIDER: E-GEOD-49434 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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PRC2 binds active promoters and contacts nascent RNAs in embryonic stem cells.

Kaneko Syuzo S Son Jinsook J Shen Steven S SS Reinberg Danny D Bonasio Roberto R

Nature structural & molecular biology 20131020 11

EZH2 is the catalytic subunit of PRC2, a central epigenetic repressor essential for development processes in vivo and for the differentiation of embryonic stem cells (ESCs) in vitro. The biochemical function of PRC2 in depositing repressive H3K27me3 marks is well understood, but how it is regulated and directed to specific genes before and during differentiation remains unknown. Here, we report that PRC2 binds at low levels to a majority of promoters in mouse ESCs, including many that are active ...[more]

PMID: 24141703

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-03-26 | E-GEOD-45489 | biostudies-arrayexpress

RNA seq in two lines of E14 mouse embryonic stem cells

Project description:This experiment was designed to obtain the polyA+ transcriptome in E14 ESCs

2013-10-19 | GSE49434 | GEO

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2013-10-19 | E-GEOD-49433 | biostudies-arrayexpress

E14 MGE

Project description:E14 MGE

| PRJNA670339 | ENA

Expression profiling of E14 cells overexpressing Hk2/Pkm2

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