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CTCF functions as a chromatin insulator in the HoxA cluster during neurogenesis [ChIP-Seq]

ABSTRACT: In this experiment, we sought to identify how the distribution of CTCF, H3K27me3, H3K4me3, Pol II, H2AK119Ub1, EZH2 3 lines (WT, Δ5|6, Δ5|6:7|9)

ORGANISM(S): Mus musculus

SUBMITTER: Danny Reinberg

PROVIDER: E-GEOD-60232 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CTCF establishes discrete functional chromatin domains at the Hox clusters during differentiation.

Narendra Varun V Rocha Pedro P PP An Disi D Raviram Ramya R Skok Jane A JA Mazzoni Esteban O EO Reinberg Danny D

Science (New York, N.Y.) 20150201 6225

Polycomb and Trithorax group proteins encode the epigenetic memory of cellular positional identity by establishing inheritable domains of repressive and active chromatin within the Hox clusters. Here we demonstrate that the CCCTC-binding factor (CTCF) functions to insulate these adjacent yet antagonistic chromatin domains during embryonic stem cell differentiation into cervical motor neurons. Deletion of CTCF binding sites within the Hox clusters results in the expansion of active chromatin into ...[more]

PMID: 25722416

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