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Expression data from 10 day old Arabidopsis thaliana seedlings

ABSTRACT: Microarrays were used to detail the global programme of gene expression comparing wild-type and RNAi knock-down plants of SPT4-1 and SPT4-2 Arabidosis seeds were stratified for 2 days and grown for 10 days on solid (0.8% Agar) MS in an plant incubator in a 16 hours day, 8 hours night rythm. Only the aerial part was harvested 2h after turning on the light.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Klaus Grasser

PROVIDER: E-GEOD-49590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The transcript elongation factor SPT4/SPT5 is involved in auxin-related gene expression in Arabidopsis.

Dürr Julius J Lolas Ihab B IB Sørensen Brian B BB Schubert Veit V Houben Andreas A Melzer Michael M Deutzmann Rainer R Grasser Marion M Grasser Klaus D KD

Nucleic acids research 20140204 7

The heterodimeric complex SPT4/SPT5 is a transcript elongation factor (TEF) that directly interacts with RNA polymerase II (RNAPII) to regulate messenger RNA synthesis in the chromatin context. We provide biochemical evidence that in Arabidopsis, SPT4 occurs in a complex with SPT5, demonstrating that the SPT4/SPT5 complex is conserved in plants. Each subunit is encoded by two genes SPT4-1/2 and SPT5-1/2. A mutant affected in the tissue-specifically expressed SPT5-1 is viable, whereas inactivatio ...[more]

PMID: 24497194

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