Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression of the mouse choroid plexus epithelium (CPE)

ABSTRACT: We investigated the gene expression of the mouse CPE. We isolated CPE cells from three mouse brains with laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays. We performed the microarrays against a common reference sample, which was mouse RPE/choroid RNA. We performed 3 mouse CPE replicates from 3 different mouse from the same strain, namely C57BL/6 .

ORGANISM(S): Mus musculus

SUBMITTER: Sarah Janssen

PROVIDER: E-GEOD-49973 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Gene expression of the human choroid plexus epithelium (CPE)

Project description:We investigated the gene expression of the human CPE.We isolated CPE cells from healthy human donor choroid plexus tissues with laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays. We performed the microarrays against a common reference sample, namely human RPE/choroid RNA. We performed 7 replicates of human CPE samples from 7 different donors.

2013-09-27 | E-GEOD-49969 | biostudies-arrayexpress

Gene expression of the choroid plexus epithelium (CPE)

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-09-27 | E-GEOD-49974 | biostudies-arrayexpress

Comparisons of the global gene profile between C-CPE treated- and untreated- SKOV-3 ovarian cancer cells.

Project description:A microarray analysis was performed to compare the global gene expression profile between C-CPE treated- and untreated- SKOV-3 ovarian cancer cells. SKOV-3 cells were treated with or without C-CPE for 72 hours, and total RNA was extracted and microarray was perfomed to compare the gene profiling changes between C-CPE treated- and untreated- cells. The experiment was performed in triplicate.

2010-06-23 | E-GEOD-22421 | biostudies-arrayexpress

Gene expression of the mouse choroid plexus epithelium (CPE)

Project description:We investigated the gene expression of the mouse CPE. We isolated CPE cells from three mouse brains with laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays.

2013-09-27 | GSE49973 | GEO

Gene expression of the human trabecular meshwork (TM) of the eye

Project description:We investigated the gene expression of the human TM. We isolated TM cells from healthy human donor eyes. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays. We performed the microarrays against a common reference sample, namely human RPE/choroid RNA. We performed 2 replicates of human TM samples from 2 different donors.

2013-09-12 | E-GEOD-50784 | biostudies-arrayexpress

RNA-Seq of native and FusionRed-transfected canine tumor cell lines after 3 hours C-CPE treatment

Project description:Claudin (CLDN) proteins are commonly expressed in cancers and accordingly targeted in novel therapeutic approaches. C-terminus of Clostridium perfringens enterotoxin (C-CPE) binds efficiently several claudins and can be used to specifically target cancer cells. This experiment used RNA-seq to profile gene expression changes of native and FusionRed transfected canine tumor cell lines;TihoDTCC0840 (0840) and TihoDProAdCarc0846 (0846) after a 3 hours of C-CPE treatment (20µg/ml).

2021-11-30 | E-MTAB-10839 | biostudies-arrayexpress

Gene expression of the pigmented and non-pigmented epithelia of the ciliary body of human healthy eyes

Project description:The ciliary body (CB) of the human eye consists of the non-pigmented (NPE) and pigmented (PE) neuro-epithelia. We investigated the gene expression of the NPE and PE, to shed light on the molecular mechanisms underlying the most important functions of the CB. Therefore we isolated NPE and PE cells from seven healthy human donor eyes using laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays. We performed the microarrays against a common reference sample, namely human RPE/choroid RNA. We performed 7 replicates of PE samples from 7 different donors and 7 NPE replicates from the same 7 different donors.

2012-09-18 | E-GEOD-37957 | biostudies-arrayexpress

Gene expression of the human choroid plexus epithelium (CPE)

2013-09-27 | GSE49969 | GEO

Cross-species genomics identifies postanatal CPE as novel choroid plexus carcinoma oncogenes.

Project description:Choroid plexus carcinomas (CPC) are poorly understood and frequently lethal brain tumors with minimal treatment options. Using a new mouse model of the disease and a large cohort of human CPCs [GSE60892; GSE60899], we performed a cross-species, genome-wide search for novel oncogenes within syntenic regions of chromosome gain. TAF12, NFYC and RAD54L, co-located on human chromosome 1p32-35.3 and mouse chromosome 4qD1-D3, were identified as oncogenes that are gained in tumors in both species and required to initiate and progress the disease in mice. TAF12 and NFYC are transcription factors that regulate the epigenome, while RAD54L plays a central role in DNA repair. Our data identify a group of concurrently gained, novel oncogenes that cooperate in the formation of CPC and unmask potential new avenues for therapy. CPC mouse model samples of tumors derived from choroid plexus epithelium (CPE) cells that are Tp53/RB/Pten null in FVB mice. Female CD1 nude (host) mice were transfected with postanatal CPE and compared with a medulloblastoma normal mouse embryonic, postnatal and adult controls (loxP TP53/RB/PTEN (no Cre)) Implanted and reimplanted are from the orthotropic transplants of the primary tumor or later tumors

2015-05-01 | E-GEOD-61659 | biostudies-arrayexpress

Carboxypeptidase E transmits its anti-migratory function in glioma cells via transcriptional regulation of cell architecture and motility regulating factors.

Project description:They aim of the study is to identify targets of Carboxypeptidase E (CPE) as well as signaling cascades that are affected by CPE which are specific for transmitting its anti-migratory effects in glioma cells by overexpressing CPE and using transcriptomics profiling of mRNAs and microRNAs.

2017-11-29 | E-MTAB-5297 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data