Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

17 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Global mRNA gene expression analysis profiling control HEK293 cell and cells expressing HSP70K71E

ABSTRACT: This is global mRNA gene expression data for HEK293 and cells expressing HSP70K71E ; most genes were down-regulated for HSP70K71E compared to the control Keywords: Global mRNA expression We overexpressed pCI1-EGFP (control) and Hsp70K71E-EGFP to HEK293 and collected total RNA from each condition to analyze global mRNA expression

ORGANISM(S): Homo sapiens

SUBMITTER: Jung Byun

PROVIDER: E-GEOD-50547 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Genome wide ChIP-Seq profiles of p300 and CBP in control and cells expressing HSP70K71E

Project description:There were more enriched p300 and CBP bindings at the promoter regions in the cells expresing HSP70K71E compared to the control HEK293 cells were overexpressed with pCI1-EGFP (control) and with HSP70K71E-EGFP (mutant) and appied for ChIP-Seq.

2013-09-24 | E-GEOD-51117 | biostudies-arrayexpress

p300 is a preferred client protein for Nuclear Chaperones

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-09-24 | E-GEOD-51118 | biostudies-arrayexpress

Effect of NPAP1/C15orf2 overexpression on transcriptome of HEK293 cells

Project description:To gain insight into the function of Nuclear pore associated protein 1 (NPAP1, formerly C15orf2), we overexpressed NPAP1 in HEK293 cells. We detected no significant difference between NPAP1-expression of induced and uninduced cells in three technical replicates, exept for an approximately 10-fold increase in the NPAP1 transcript itself. This indicates that overexpression of NPAP1 does not change mRNA expression profiles of HEK293 cells. We used microarrays to investigate global gene expression changes depending on the level of NPAP1/C15orf2 We compared NPAP1 overexpressing cells to untreated cells, which do not express detectable amounts of NPAP1 protein, to determine global gene expression changes.

2012-06-17 | E-GEOD-36575 | biostudies-arrayexpress

Expression data from human HEK293 cells

Project description:LPA, which can signal through the Ga12/13 family of G proteins, is a potent stimulator of fibroblast migration. LPA downregulate mTOR activity dependent on protein synthesis in cell migration and bleomycin-induced lung fibrosis. We used microarray to identify LPA-regualted gene expression and find potent regulators in this process. HEK293 cells were treated with or without LPA. Then RNA was extracted and Hybridization was done on Affymetrix microarrays.

2012-01-14 | E-GEOD-35084 | biostudies-arrayexpress

Global mRNA gene expression analysis profiling control HEK293 cell and cells expressing HSP70K71E

Project description:This is global mRNA gene expression data for HEK293 and cells expressing HSP70K71E ; most genes were down-regulated for HSP70K71E compared to the control Keywords: Global mRNA expression

2013-09-24 | GSE50547 | GEO

TPC1 interactome from HEK293 cells

Project description:TPC1 interactome data obtained by affinity precipitation of TPC1-eGFP-FLAG with anti-FLAG antibody from HEK293 cells. Heavily labeled proteins were from cells expressing TPC1-eGFP-FLAG and lightly labeled proteins were from cells expressing eGFP-FLAG. Proteins were eluted from beads by FLAG peptide.

2021-05-12 | MSV000087417 | MassIVE

TPC2 interactome from HEK293 cells

Project description:TPC2 interactome data obtained by affinity precipitation of TPC2-eGFP-FLAG with anti-FLAG antibody from HEK293 cells. Heavily labeled proteins were from cells expressing TPC2-eGFP-FLAG and lightly labeled proteins were from cells expressing eGFP-FLAG. Proteins were eluted from beads by FLAG peptide.

2021-05-12 | MSV000087418 | MassIVE

Expression profile of HEK293 cells stably transfected with PRL-1 (PTP4A1) or empty pcDNA4 vector (control)

Project description:The multifunctional protein tyrosine phosphatase PRL-1 (Gene Symbol: PTP4A1) has been identified as an important oncogene with roles in promoting cell proliferation, survival, migration, invasion, and metastasis. However, little is currently known about the signaling pathways through which it mediates its effects. We used Affymetrix Human Genome U133 Plus 2.0 arrays to examine the global molecular changes of gene expression occurring in response to stable PRL-1 overexpression in human embryonic kidney 293 (HEK293) cells. Total RNA was extracted from triplicate cultures of HEK293 cells stably overexpressing PRL-1 (HEK293-PRL-1) and HEK293 cells stably transfected with empty pcDNA4 vector (HEK293-Vector). Samples were hybridized to Affymetrix HG U133 Plus 2.0 microarrays (1 array per sample for a total of 6 arrays). One of the arrays hybridized with a PRL-1 transfected sample was excluded after qRT-PCR demonstrated that this sample did not exhibit increased PRL-1 expression over the level of the empty vector controls. The remaining 2 HEK293-PRL-1 samples both expressed PRL-1 at least 2-fold higher than any of the 3 empty vector controls.

2013-07-30 | E-GEOD-42586 | biostudies-arrayexpress

ChIP-seq from HEK293 (ENCSR371LLY)

Project description:eGFP-ZNF37A ChIP-seq on human HEK293 cells. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2019-03-03 | GSE127613 | GEO

ChIP-seq from HEK293 (ENCSR125ULS)

Project description:eGFP-ZNF561 ChIP-seq on human HEK293 cells. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2019-03-03 | GSE127418 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data