Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

TPC2 interactome from HEK293 cells

ABSTRACT: TPC2 interactome data obtained by affinity precipitation of TPC2-eGFP-FLAG with anti-FLAG antibody from HEK293 cells. Heavily labeled proteins were from cells expressing TPC2-eGFP-FLAG and lightly labeled proteins were from cells expressing eGFP-FLAG. Proteins were eluted from beads by FLAG peptide.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jiusheng Yan

PROVIDER: MSV000087418 | MassIVE | Wed May 12 22:52:00 BST 2021

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

TPC1 interactome from HEK293 cells

Project description:TPC1 interactome data obtained by affinity precipitation of TPC1-eGFP-FLAG with anti-FLAG antibody from HEK293 cells. Heavily labeled proteins were from cells expressing TPC1-eGFP-FLAG and lightly labeled proteins were from cells expressing eGFP-FLAG. Proteins were eluted from beads by FLAG peptide.

2021-05-12 | MSV000087417 | MassIVE

NAADP interactome from HEK293 cells expressing exogenous TPC2 channel

Project description:NAADP interactome data obtained by affinity precipitation with immobilized NAADP from HEK293 cells expressing exogenous TPC2-eGFP-FLAG. Heavily labeled proteins were pulled-down by immobilized NAADP and lightly labeled proteins were pulled-down by empty beads (no NAADP). Proteins were eluted from beads by NAADP first (raw data file names contain NAADP) and then by SDS (file names contain SDS). The suffix 1-3 for file names indicates order of excised gel bands from top.

2021-05-12 | MSV000087416 | MassIVE

NAADP interactome from HEK293 cells expressing exogenous TPC1 channel

Project description:NAADP interactome data obtained by affinity precipitation with immobilized NAADP from HEK293 cells expressing exogenous TPC1-eGFP-FLAG. Heavily labeled proteins were pulled-down by immobilized NAADP and lightly labeled proteins were pulled-down by empty beads (no NAADP). Proteins were eluted from beads by NAADP first (raw data file names contain NAADP) and then by SDS (file names contain SDS). The suffix 1-3 for file names indicates order of excised gel bands from top.

2021-05-12 | MSV000087415 | MassIVE

PALB2 interactome in asynchronous human cells

Project description:Identification of interacting partners of the Partner and Localizer of BRCA2 (PALB2), essential regulator of DNA repair by homologous recombination. Interacting partners of PALB2 were purified by GFP pull down from asynchronous HEK293 Flp-In T-REx cells, exogenously expressing Flag-EGFP tagged PALB2 at a similar level than the endogenous PALB2 level. Before GFP pull down, whole cell protein extracts were pre-cleared on IgG agarose beads to decrease binding of non-specific proteins during GFP pull down. GFP pull down were performed using GFP-Trap agarose beads (Chromotek) and washed at low salt concentration (150mM NaCl), to maintain interactions of low affinity binding protein partners. As a negative control, Flag-EGFP interacting proteins were purified, following the same protocol as described for the purification of Flag-EGFP PALB2 interacting proteins. Experiments were performed in triplicate.

2017-07-18 | PXD006391 | Pride

Chromatin immunoprecipitation-sequencing of HEK293 cells (HEK293-HPT-blue) and HEK293 cells stably over-expressing the BAHD1 gene (HEK293-HPT-BAHD1)

Project description:We report the genomic occupancy of the BAHD1 protein in HEK293 cells over-expressing the BAHD1 gene (HEK293-HPT-BAHD1) We used Native ChIP-seq to identify DNA regions bound to BAHD1 (native ChIP involves use of native chromatin before precipitation of immune complexes, without formaldehyde crosslinking of proteins with nucleic acids)

2015-12-11 | E-GEOD-53372 | biostudies-arrayexpress

Immunoprecipitated proteins associated with foci-forming Eno2p-EGFP-FLAG or non-forming Eno2V22Ap-EGFP-FLAG in yeast Saccharomyces cerevisiae under hypoxia

Project description:Yeast enolase (Eno2p) conjugated with EGFP and Flag-tag (Eno2p-EGFP-FLAG) and Eno2p with V22A substitution (Eno2V22Ap) conjugated with EGFP and Flag-tag (Eno2V22Ap-EGFP-FLAG) were produced in baker's yeast S. cerevisiae. After semi-anaerobic culture at 30 ˚C for 12h, cells producing Eno2p-EGFP-FLAG formed fluorescent foci, while cells producing Eno2V22Ap-EGFP-FLAG did not. The cells were collected and lysed, and proteins Eno2p-EGFP-FLAG or Eno2V22Ap-EGFP-FLAG and the associated proteins were coimmunoprecipitated using ANTI-FLAG M2 affinity gel and analyzed. Data contain two biological replicates and two technical replicates (n = 4). As the results, 96 proteins were detected with both recombinant Eno2p-EGFP-FLAG and Eno2V22Ap-EGFP-FLAG protein, 29 proteins were detected only with recombinant Eno2p-EGFP-FLAG protein, and 16 proteins were detected only with recombinant Eno2V22Ap-EGFP-FLAG protein. Data Processing/Data Analysis: The separated analytes were detected on an LTQ Velos linear ion trap mass spectrometer (Thermo Scientific). For data-dependent acquisition, the method was set to automatically analyze the five most intense ions observed in the MS scan. The mass spectrometry data were used for protein identification by the Mascot search engine on Protein Discoverer software (ver. 1.2, Thermo Scientific) against the information in the Saccharomyces Genome Database (SGD; http://www.yeastgenome.org). Search parameters for peptide identification included a precursor mass tolerance of 1.2 Da, a fragment mass tolerance of 0.8 Da, a minimum of one tryptic terminus, and a maximum of one internal trypsin cleavage site. Cysteine carbamidomethylation (+57.021 Da) and methionine oxidation (+15.995 Da) were set as a differential amino acid modification. The data were then filtered at a q value ≤ 0.01 corresponding to 1% FDR at the spectral level.

2014-02-25 | PXD000173 | Pride

Phosphoproteome of PINK1 in HEK293 cells

Project description:triple SILAC phosphoproteomics experiment of Flp-In T-Rex HEK293 cells stably expressing either FLAG empty, PINK1-FLAG Kinase dead or PINK1-FLAG wild type were grown in ‘light’ (K0R0), ‘medium’ (K4R6) and ‘heavy’ (K8,R10) SILAC media, respectively.

2015-09-16 | PXD002127 | Pride

ChIP-Seq of SMARCAD1 and KAP1 in male mouse embryonic stem cells (E14)

Project description:The ATP dependent chromatin remodeler SMARCAD1 and the co-repressor KAP1 (Trim28/Tif1 beta) interact directly. We wanted to understand the interplay between these two proteins in the context of chromatin; in particular at repetitive sequences. Therefore, we carried out an investigation in E14 ES cells of the genome wide binding behaviour of KAP1 and FLAG-tagged SMARCAD1; both wild-type SMARCAD1 and a catalytically inactive mutant. The E14 cells analysed were either wild-type or depleted for endogenous SMARCAD1 protein. FLAG and KAP1 ChIP was performed on double-cross linked chromatin and sequenced on an Illumina HiSeq1500 with matched input controls. A FLAG antibody precipitation was carried out on cells not expressing FLAG tagged protein as an additional control.

2019-02-17 | E-MTAB-7011 | biostudies-arrayexpress

FLAG-PHF3 interactome in HEK293 cells

Project description:FLAG-hPHF3 was transiently expressed in human HEK293 cells, followed by anti-FLAG immunoprecipitation, on-bead digest and NanoLC-MS analysis.

2022-02-17 | PXD026292 | Pride

Characterization of RBM10 interactome in unchallenged and MK1775-treated cells

Project description:Mapping of RBM10-interactome using GFP-trap purification of EGFP-RBM10 expressed in HEK293T cells before and after WEE1 inhibition by MK1775. HEK293T expressing EGFP only or EGFP-RBM10 fusion (2 main isoforms) were left untreated or treated with 300nM MK1775 for 4 hours prior to GFP-trap enrichment and mass spectrometry analysis.

2024-07-16 | PXD040109 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data