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METTL3 and WTAP

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: sun baofa

PROVIDER: E-GEOD-50583 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase.

Ping Xiao-Li XL Sun Bao-Fa BF Wang Lu L Xiao Wen W Yang Xin X Wang Wen-Jia WJ Adhikari Samir S Shi Yue Y Lv Ying Y Chen Yu-Sheng YS Zhao Xu X Li Ang A Yang Ying Y Dahal Ujwal U Lou Xiao-Min XM Liu Xi X Huang Jun J Yuan Wei-Ping WP Zhu Xiao-Fan XF Cheng Tao T Zhao Yong-Liang YL Wang Xinquan X Rendtlew Danielsen Jannie M JM Liu Feng F Yang Yun-Gui YG

Cell research 20140110 2

The methyltransferase like 3 (METTL3)-containing methyltransferase complex catalyzes the N6-methyladenosine (m6A) formation, a novel epitranscriptomic marker; however, the nature of this complex remains largely unknown. Here we report two new components of the human m6A methyltransferase complex, Wilms' tumor 1-associating protein (WTAP) and methyltransferase like 14 (METTL14). WTAP interacts with METTL3 and METTL14, and is required for their localization into nuclear speckles enriched with pre- ...[more]

PMID: 24407421

Similar Datasets

Binding motif analysis of METTL3，WTAP in METTL3，WTAP overexpressed Human 293T cells

Project description:label the cells overexpressed Myc tagged METTL3 and Flag tagged WTAP with 4-SU, the RNA bound by METT3,WTAP can be got by Myc or Flag IP followed by RNA isolation by using the TRIzol (Invitrogen) reagent by following the company manual.the RNA was then used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.The libraries were sequenced using HiSeq2000 (Illumina) in single-read mode, creating reads with a length of 50 bp. Sequencing chemistry v2 (Illumina) was used and samples were multiplexed in two samples per lane. discovery of the binding motif of METTL3，WTAP in METTL3，WTAP overexpressed Human 293T cells

2014-01-16 | E-GEOD-50580 | biostudies-arrayexpress

Transcriptomics analysis of gene expression in normal and METTL3 or WTAP deficient Human 293T cells

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2013-11-29 | E-GEOD-46705 | biostudies-arrayexpress

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2021-07-16 | PXD024326 | Pride

Transcriptomics analysis of gene expression in normal and YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 or SRSF10 deficient human HeLa cells

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2016-02-17 | E-GEOD-71095 | biostudies-arrayexpress

m6a peak analysis in normal, FTO deficient and five stages of adipogenesis (D-2/0/2/5/10) in Mouse embryo fibroblast 3T3-L1 pre-adipocytes

Project description:Total RNA was extracted using TRI® Reagent (Sigma). cDNA was synthesized by RevertAid™ First Strand cDNA Synthesis Kit with Oligo dT primers (K1622, Fermentas) following manufacturer’s recommendations. PCR reactions were carried out on a DNAEngine® Thermal Cycler (PTC-0200G, Bio-Rad) in 25 μl reaction volume containing 1 μl cDNA, 200 nM primer pairs and components of TaKaRa Taq™ kit (R001A, Takara). All samples were analyzed in triplicate RT-qPCR.mRNAs were extracted using biotinylated poly(dT) oligo, followed by further removing of contaminated rRNA using RiboMinus Transcriptome Isolation Kit (K1550-02, Invitrogen). Then mRNAs were fragmented into 100-200nt length and subjected to immunoprecipitation with m6A specific antibody.The libraries were sequenced using HiSeq2000 (Illumina) in single-read mode, creating reads with a length of 101 bp. Sequencing chemistry v2 (Illumina) was used and samples were multiplexed in two samples per lane. discovery of the binding motif of m6a in normal, FTO deficient and five stages of adipogensis (D-2/0/2/5/10) in Mouse embryo ﬁbroblast 3T3-L1 pre-adipocytes

2015-03-09 | E-GEOD-53244 | biostudies-arrayexpress

Targeted Quantitative Profiling of Epitranscriptomic Reader, Writer and Eraser Proteins Revealed Potential Crosstalk between N6-Methyladenosine and Other RNA Modifications

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Interaction between TBP and condensing drives the organization and faithful segregation of mitotic chromosomes

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