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Lympho-myeloid contribution of an immune-restricted progenitor emerging prior to definitive hematopoietic stem cells

ABSTRACT: An immune-restricted lymphomyeloid-primed progenitor with the capacity to contribute to both myeloid and lymphoid lineages in the developing embryo emerges prior to definitive HSCs. Examination of fetal sorted lymphoid primed progentors and adult progenitors The fastq files are not provided at this time due to further analyses.

ORGANISM(S): Mus musculus

SUBMITTER: Supat Thongjuea

PROVIDER: E-GEOD-50896 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Lymphomyeloid contribution of an immune-restricted progenitor emerging prior to definitive hematopoietic stem cells.

Böiers Charlotta C Carrelha Joana J Lutteropp Michael M Luc Sidinh S Green Joanna C A JC Azzoni Emanuele E Woll Petter S PS Mead Adam J AJ Hultquist Anne A Swiers Gemma G Perdiguero Elisa Gomez EG Macaulay Iain C IC Melchiori Luca L Luis Tiago C TC Kharazi Shabnam S Bouriez-Jones Tiphaine T Deng Qiaolin Q Pontén Annica A Atkinson Deborah D Jensen Christina T CT Sitnicka Ewa E Geissmann Frederic F Godin Isabelle I Sandberg Rickard R de Bruijn Marella F T R MF Jacobsen Sten Eirik W SE

Cell stem cell 20130919 5

In jawed vertebrates, development of an adaptive immune-system is essential for protection of the born organism against otherwise life-threatening pathogens. Myeloid cells of the innate immune system are formed early in development, whereas lymphopoiesis has been suggested to initiate much later, following emergence of definitive hematopoietic stem cells (HSCs). Herein, we demonstrate that the embryonic lymphoid commitment process initiates earlier than previously appreciated, prior to emergence ...[more]

PMID: 24054998

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Lympho-myeloid contribution of an immune-restricted progenitor emerging prior to definitive hematopoietic stem cells

Project description:An immune-restricted lymphomyeloid-primed progenitor with the capacity to contribute to both myeloid and lymphoid lineages in the developing embryo emerges prior to definitive HSCs.

2013-11-26 | GSE50896 | GEO

Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells

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Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin

Project description:Studies of adult human hematopoiesis have until now relied on the expression of CD10 to define lymphoid commitment. We report a novel lymphoid-primed population in human bone marrow that is generated from hematopoietic stem cells (HSC) prior to the onset of CD10 expression and B cell commitment, and is identified by high levels of the homing molecule L-selectin (CD62L). CD10-CD62Lhi progenitors have full lymphoid (B/T/NK) potential, and show reduced myeloid and absent erythroid potential. Genome-wide gene expression analysis demonstrates that the CD10-CD62Lhi population represents an intermediate stage of differentiation between CD34+CD38- HSC and CD34+lin-CD10+ progenitors marked by down-regulation of TAL1 and MPL, upregulation of E2A, CD3E and IL2RG expression, and absent B cell commitment or RAG1/2 expression. Immature CD34+CD1a- thymocytes are also CD62Lhi and L-selectin ligands are expressed at the cortico-medullary junction, suggesting a possible role for L-selectin in human thymic homing. These studies identify the earliest stage of lymphoid priming in human bone marrow. Freshly harvested human bone marrow was ficoll purified, enriched for CD34+, and then FACS sorted. It was then sorted into 3 samples CD34+CD38-, CD34+ CD10- CD62L++, and CD34+ CD10+, with 3 independent biological replicates

2012-08-14 | E-GEOD-35685 | biostudies-arrayexpress

Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin

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Dach1 downregulation marks a lymphoid-primed progenitor in early haematopoiesis (2X96 cells)

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Mus musculus

Project description:Lyl-1 knockout vs wildtype Lymphoid Primed Multipotent Progenitors (LMPPs)

| PRJNA168481 | ENA

Foxd3 promotes the exit from naïve pluripotency and prevents germline specification through enhancer decommissioning [RNA-Seq]

Project description:Following implantation, mouse epiblast cells transit from a naïve to a primed state in which they are competent for both somatic and primordial germ cell (PGC) specification. Using mouse embryonic stem cells (mESC) as an in vitro model to study the transcriptional regulatory principles orchestrating peri-implantation development, here we show that the transcription factor Foxd3 is necessary for the exit from naïve pluripotency and the progression to a primed pluripotent state. During this transition, Foxd3 acts as a repressor that dismantles a significant fraction of the naïve pluripotency expression program through the decommissioning of active enhancers associated with key naïve pluripotency and early germline genes. Subsequently, Foxd3 needs to be silenced in primed pluripotent cells to allow the reactivation of relevant genes required for proper PGC specification. Our findings uncover a wave of activation-deactivation of Foxd3 as a crucial step for the exit from naïve pluripotency and subsequent PGC specification. mRNA profiles were generated by RNA-seq in duplicates for each of the following mESC lines: Foxd3fl/fl;Cre-ER mESC maintained in "Serum+LIF" (SL) treated with TM for three days (SL Foxd3-/-); untreated Foxd3fl/fl;Cre-ER SL mESC (SL Foxd3fl/fl); tetON Foxd3 SL mESC treated with Dox for three days; WT SL mESC treated with Dox for three days; Foxd3fl/fl;Cre-ER mESC maintained in "2i+LIF" (2i) treated with TM for three days (2i Foxd3-/-); untreated Foxd3fl/fl;Cre-ER 2i mESC (2i Foxd3fl/fl).

2015-09-14 | E-GEOD-70546 | biostudies-arrayexpress

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