Unknown,Transcriptomics,Genomics,Proteomics

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The Gene Expression Profile of Peripheral Blood Mononuclear Cells from EV71-Infected Rhesus Neonates

ABSTRACT: To study the gene expression during EV71 infection, we have employed whole Rhesus Monkey genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish infection process. Rhesus monkey peripheral blood from the experiment or control donors was isolated, extracted the RNA and screened by microarray. The results indicated that the expression levels of more than 500 functional genes associated with multiple pathways were modulated. To confirm the results of the microarray analysis, 25 individual genes were random selected and were analyzed using real-time RT-PCR amplification. The expression levels of genes associated with immune inflammatory responses were up-regulated during the period from day 4 to 10 post-infection. The expressions of two genes (TAC1 and IL17A), which play major roles in inflammatory reactions, were remarkably up-regulated during the infection period. Enterovirus 71 infection induced gene expression in rhesus monkey peripheral blood mononuclear cells was measured at 4, 7 and 10 days after viral infection. Three samples from two experiment donors and one control donor were performed at each time (4, 7 or 10 days). To exclude the individual difference, mix the two experiment samples at each time, and mix the three control samples.

ORGANISM(S): Macaca mulatta

SUBMITTER: Ying Zhang

PROVIDER: E-GEOD-51103 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Embryonic stem cells (ESCs) may be able to cure or alleviate the symptoms of various degenerative diseases. However, unresolved issues regarding apoptosis, maintaining function and tumor formation mean a prudent approach should be taken towards advancing ESCs into human clinical trials. The rhesus monkey provides the ideal model organism for developing strategies to prevent immune rejection and test the feasibility, safety and efficacy of ESC-based medical treatments. Transcriptional profiling of rhesus ESCs provides a foundation for future pre-clinical ESC research using non-human primates as the model organism. In this research we use microarray, immunocytochemistry, real-time and standard RT-PCR to characterize and transcriptionally profile rhesus monkey embryonic stem cells. We identify 367 rhesus monkey stemness genes, we demonstrate the high level (>85%) of conservation of rhesus monkey stemness gene expression across five different rhesus monkey embryonic stem cell lines, we demonstrate that rhesus monkey ESC lines maintain a pluripotent undifferentiated state over a wide range of Pou5f1 (Oct-4) expression levels and we compare rhesus monkey, human and murine stemness genes to identify the key mammalian stemness genes. The supplementary tables list the genes that have been upregulated in each undifferentiated rhesus monkey embryonic stem cell line (GSM99998, GSM99999,GSM100000, GSM100001, GSM100002, GSM99965, GSM99966) in comparison analysis with the pooled differentiated embryonic stem cells (GSM99840). Supplemental Table 1 contains the comparison analysis for all 52,865 probe sets on the rhesus monkey gene chip, Supplemental Table 2 contains the rhesus monkey genes that were significantly upregulated (FC>3) in the ORMES-6 biological replicates, Supplemental Table 3 contains the rhesus monkey genes that were significantly upregulated (FC>3) in the pooled differentiated EBs and Supplemental Tables 4-8 represent genes that were significantly upregulated in ORMES 6A, 7, 9, 10 and 13 respectively. Supplemental Table 9 contains the RT-PCR primers used in this project. Keywords: Rhesus monkey embryonic stem cell microarray

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The Gene Expression Profile of Peripheral Blood Mononuclear Cells from EV71-Infected Rhesus Neonates

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