Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq transcriptome profiling of equine inner cell mass and trophectoderm


ABSTRACT: Transcriptomic analysis of ICM and TE from in vivo-derived equine blastocysts using Illumina sequencing technology RNA was extracted from individual equine blastocyst ICM and TE (Arcturus Picopure), cDNA was synthesized and amplified (Nugen Ovation V2) and indexed libraries were created for sequencing (TruSeq DNA V1)

ORGANISM(S): Equus caballus

SUBMITTER: James Chitwood 

PROVIDER: E-GEOD-51431 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

RNA-seq transcriptome profiling of equine inner cell mass and trophectoderm.

Iqbal Khursheed K   Chitwood James L JL   Meyers-Brown Geraldine A GA   Roser Janet F JF   Ross Pablo J PJ  

Biology of reproduction 20140320 3


Formation of the inner cell mass (ICM) and trophectoderm (TE) marks the first differentiation event in mammalian development. These two cell types have completely divergent fates for the remainder of the developmental process. The molecular mechanisms that regulate ICM and TE formation are poorly characterized in horses. The objective of this study was to establish the transcriptome profiles of ICM and TE cells from horse blastocysts using RNA sequencing (RNA-seq). A total of 12 270 genes were f  ...[more]

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