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RNA sequencing of mouse embryonic day 3.5 blastocyst inner cell mass cells

ABSTRACT: Inner cell mass (ICM) cells of two independent E3.5 blastocysts were profiled by transcriptome sequencing to assess the expression of extracellular matrix components.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Paul Bertone

PROVIDER: E-MTAB-2555 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification.

Boroviak Thorsten T Loos Remco R Loos Remco R Bertone Paul P Smith Austin A Nichols Jennifer J

Nature cell biology 20140525 6

The precise relationship of embryonic stem cells (ESCs) to cells in the mouse embryo remains controversial. We present transcriptional and functional data to identify the embryonic counterpart of ESCs. Marker profiling shows that ESCs are distinct from early inner cell mass (ICM) and closely resemble pre-implantation epiblast. A characteristic feature of mouse ESCs is propagation without ERK signalling. Single-cell culture reveals that cell-autonomous capacity to thrive when the ERK pathway is i ...[more]

PMID: 24859004

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