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Transcription profiling of rice seedlings after hormone treatment

ABSTRACT: Indica rice seedlings of IR64 variety were grown hydroponically for 7-days in a culture room with a daily photoperiodic cycle of 14h light and 10h dark. Seedlings were incubated in 0.1% dimethyl sulfoxide (control) or 50 micromolar solutions of indole-3-acetic acid (IAA treatment) and benzyl aminopurine (BAP treatment) for 1h and 3h. Equal amounts of 1h and 3h samoles were pooled for each treatment before RNA isolation. The 5 micrograms of each total RNA sample was processed for microarray analysis according to Affymetrix protocol. Experiment Overall Design: Two biological replicates for each sample (control, IAA treated, BAP treated) were used for microarray analysis. The experiment involves the identification of differentially expressed genes in IAA and BAP treated samples as compared to control.

ORGANISM(S): Oryza sativa

SUBMITTER: Mukesh Jain

PROVIDER: E-GEOD-5167 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice.

Jain Mukesh M Khurana Jitendra P JP

The FEBS journal 20090428 11

Auxin influences growth and development in plants by altering gene expression. Many auxin-responsive genes have been characterized in Arabidopsis in detail, but not in crop plants. Earlier, we reported the identification and characterization of the members of the GH3, Aux/IAA and SAUR gene families in rice. In this study, whole genome microarray analysis of auxin-responsive genes in rice was performed, with the aim of gaining some insight into the mechanism of auxin action. A comparison of expre ...[more]

PMID: 19490115

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