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Low Dose PDT - Human Cells

ABSTRACT: Human tumour cell lines PC3, DU145, U87 and U373 (prostate carcinoma and glioblastoma) were treated with photosensitizers 5-aminolaevulinic acid (5-ALA) or photofrin. Then, the cells were irradiated sublethally with 635 nm laser light.
After photodynamic therapy, the cells were grown at 37°C for 4 or 24 hours in the dark until extraction of total RNA and expression profiling.

ORGANISM(S): Homo sapiens

SUBMITTER: Alexander Buchner

PROVIDER: E-MEXP-3020 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Induction of immune mediators in glioma and prostate cancer cells by non-lethal photodynamic therapy.

Kammerer Robert R Buchner Alexander A Palluch Patrick P Pongratz Thomas T Oboukhovskij Konstantin K Beyer Wolfgang W Johansson Ann A Stepp Herbert H Baumgartner Reinhold R Zimmermann Wolfgang W

PloS one 20110630 6

<h4>Background</h4>Photodynamic therapy (PDT) uses the combination of photosensitizing drugs and harmless light to cause selective damage to tumor cells. PDT is therefore an option for focal therapy of localized disease or for otherwise unresectable tumors. In addition, there is increasing evidence that PDT can induce systemic anti-tumor immunity, supporting control of tumor cells, which were not eliminated by the primary treatment. However, the effect of non-lethal PDT on the behavior and malig ...[more]

PMID: 21738796

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