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Arabidopsis EDM2 Promotes IBM1 Distal Polyadenylation and Regulates Genome DNA Methylation Patterns [RNA-Seq]

ABSTRACT: A forward genetics screen led to the identification of the putative chromatin regulator EDM2 as a cellular anti-silencing factor and regulator of genome DNA methylation patterns. EDM2 contains a composite PHD finger domain that recognizes both active H3K4 and repressive H3K9 methylation marks at the intronic repeat elements in genes such as the histone H3K9 demethylase gene IBM1, and is necessary for maintaining the expression of these genes by promoting mRNA distal polyadenylation. Because of its role in maintaining IBM1 expression, EDM2 is required for preventing CHG methylation in the bodies of thousands of genes.Our results thus increase the understanding of anti-silencing, genome methylation patterns, and regulation of alternative RNA processing by intronic heterochromatin. Col-0 and edm2-4 total RNA are extracted from leaves and then polyA mRNAs are isolated for mRNA-seq.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Lan Yang 

PROVIDER: E-GEOD-51763 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Arabidopsis EDM2 promotes IBM1 distal polyadenylation and regulates genome DNA methylation patterns.

Lei Mingguang M   La Honggui H   Lu Kun K   Wang Pengcheng P   Miki Daisuke D   Ren Zhizhong Z   Duan Cheng-Guo CG   Wang Xingang X   Tang Kai K   Zeng Liang L   Yang Lan L   Zhang Heng H   Nie Wenfeng W   Liu Pan P   Zhou Jianping J   Liu Renyi R   Zhong Yingli Y   Liu Dong D   Zhu Jian-Kang JK  

Proceedings of the National Academy of Sciences of the United States of America 20131118 1

DNA methylation is important for the silencing of transposons and other repetitive elements in many higher eukaryotes. However, plant and mammalian genomes have evolved to contain repetitive elements near or inside their genes. How these genes are kept from being silenced by DNA methylation is not well understood. A forward genetics screen led to the identification of the putative chromatin regulator Enhanced Downy Mildew 2 (EDM2) as a cellular antisilencing factor and regulator of genome DNA me  ...[more]

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