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PHF19 isoforms induced gene expression alteration in colon cancer cells

ABSTRACT: This experiment is designed to evaluate gene expression alteration following silencing PHF19 isoforms in colon cancer cells. Total RNA were extraced from indicated stable cell lines, two biological replications for each treatment.

ORGANISM(S): Homo sapiens

SUBMITTER: Mengfeng Li

PROVIDER: E-GEOD-52019 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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SMAD2/3 mediated transcripts in A549 cells

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Antagonizing miR-128 abrogates chemoresistance-associated metastasis and is therapeutic in non-small cell lung cancer [mRNA]

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MiR-374a Promotes Epithelial-Mesenchymal Transition (EMT) and Metastasis of Breast Cancer (mRNA dataset)

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Phf19 knockdown effect on F9 embryonal carcinoma cells

Project description:PCL family protein Phf19/Pcl3 is one of the accessory components of the PRC2 core complex, and Phf19 is highly expressed in murine ES cells and an ES cell-like embryonic carcinoma cell line, F9 cells. Here we performed microarray analysis of embryonal carcinoma cell line F9 following Phf19 knockdown by shRNA. Knocking down Phf19/Pcl3 in F9 embryonic cells led to derepression of numerous PRC2 direct target genes. 4 sampels including 2 shRNA vector control cell lines and 2 shPhf19 cell lines were used for RNA extraction and Affymetrix mouse 430 2.0 arrays.

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Phf19 knockdown effect on F9 embryonal carcinoma cells

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Phf19 links methylated lysine 36 of histone H3 to regulation of Polycomb activity [ChIP-Seq]

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2012-10-16 | E-GEOD-41589 | biostudies-arrayexpress

The role of PHF19 in promoting H3K27me3 deposition in multiple myeloma (ChIP-seq)

Project description:We here report a PRC2-associated cofactor, PHD finger protein 19 (PHF19, also known as Polycomb-like 3), as a crucial mediator of tumorigenicity in multiple myeloma (MM). Using various MM models, we demonstrated a critical requirement of PHF19 for tumor growth in vitro and in vivo. Mechanistically, PHF19-mediated oncogenic effect relies on its PRC2-interacting and chromatin-binding functions. ChIP-Seq profiling showed a critical role for PHF19 in maintaining the H3K27me3 landscape. PHF19 depletion led to loss of broad H3K27me3 domains possibly due to impaired H3K27me3 spreading from CpG islands

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