Signature of human hepatocytes treated with Aroclor1254
Ontology highlight
ABSTRACT: In order to determine molecular rules for transcriptional regulation of targeted genes to explain in part the pleiotropic effect observed in animals and humans upon exposure to Aroclor 1254 we treated human hepatocytes with Aroclor1254 and analysed RNA expression using a Nimblegene human custom array. Human hepatocytes isolated from surgical material were treated with 20µM Aroclor1254 for 72h. Non-treated cells served as control. Total RNA was isolated using QIAGEN´s RNeasy total RNA isolation kit according to the manufacturerâs recommendations. Oligonucleotide microarrays were hybridized and washed according to the manufacturerâs recommendations.
ORGANISM(S): Homo sapiens
SUBMITTER: Jürgen Borlak
PROVIDER: E-GEOD-5213 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
ACCESS DATA