Global gene expression profiling of endothelial colony-forming cells from term and preterm neonates
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ABSTRACT: Endothelial colony-forming cells were isolated and expanded from the mononuclear cell fraction (MNC) obtained from the cord blood of term (CT) and preterm (PT) neonates, and characterized as previously described (Vassallo PF et al 2014). The objective of the study was to identify differentially expressed genes between CT and PT neonates. ECFC were harvested from cultures dishes at passage 3 and total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion), according to the manufacturerâs recommendations. Cy3-CTP labeled RNA was prepared according to standard Agilent protocol from 400ng total RNA. The hybridization was performed for 17 hrs at 65°C. cRNA labeling and hybridization performance were performed and all parameters checked were found within the manufacturers specifications. Arrays were scanned as described in the manufacturersâ protocol. Signal intensities on 20 bit tiff images were calculated by Feature Extraction software (FE, Version 8.5; Agilent Technologies). Data analyses were conducted with GeneSpring GX software (Vers.13.1.1; Agilent Technologies)
INSTRUMENT(S): Agilent Microarray Hybridization Chamber Kit and Hybridization oven rotator
ORGANISM(S): Homo sapiens
SUBMITTER: Stéphanie Simoncini
PROVIDER: E-MTAB-4860 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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