Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Met Expression Signature in Hepatocytes

ABSTRACT: To identify HGF/Met regulated genes, we performed expression microarray analysis after inducible activation of Met receptor in primary cultures of hepatocytes established from wild-type control (Alb-Cre +/-) and Met conditional knockout mice (Alb-Cre +/-; Met Fl/Fl). Total RNA was isolated from untreated hepatocyte cultures as well as from cultures treated with 50 ng/ml of HGF for 0.5, 2, 12 or 24 hours. RNA collected from these experiments was converted to fluorescently labeled cDNA and used for hybridizations of oligonucleotide microarrays. All experiments were repeated in triplicates. Total RNA from pooled wild-type mouse hepatocytes was used as universal reference and all hybridizations were repeated following a reverse flourescing.

ORGANISM(S): Mus musculus

SUBMITTER: Snorri Thorgeirsson

PROVIDER: E-GEOD-4451 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Met-regulated expression signature defines a subset of human hepatocellular carcinomas with poor prognosis and aggressive phenotype.

Kaposi-Novak Pal P Lee Ju-Seog JS Gòmez-Quiroz Luis L Coulouarn Cédric C Factor Valentina M VM Thorgeirsson Snorri S SS

The Journal of clinical investigation 20060518 6

Identification of specific gene expression signatures characteristic of oncogenic pathways is an important step toward molecular classification of human malignancies. Aberrant activation of the Met signaling pathway is frequently associated with tumor progression and metastasis. In this study, we defined the Met-dependent gene expression signature using global gene expression profiling of WT and Met-deficient primary mouse hepatocytes. Newly identified transcriptional targets of the Met pathway ...[more]

PMID: 16710476

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Dataset Information

Met Expression Signature in Hepatocytes

Publications

Met-regulated expression signature defines a subset of human hepatocellular carcinomas with poor prognosis and aggressive phenotype.

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