Improvements in sample preparation for ChIP-chip assays
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Peggy Farnham
PROVIDER: E-GEOD-5251 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Comparison of sample preparation methods for ChIP-chip assays.
O'Geen Henriette H Nicolet Charles M CM Blahnik Kim K Green Roland R Farnham Peggy J PJ
BioTechniques 20061101 5
A single chromatin immunoprecipitation (ChIP) sample does not provide enough DNA for hybridization to a genomic tiling array. A commonly used technique for amplifying the DNA obtained from ChIP assays is ligation-mediated PCR (LM-PCR). However; using this amplification method, we could not identify Oct4 binding sites on genomic tiling arrays representing 1% of the human genome (ENCODE arrays). In contrast, hybridization of a pool of 10 ChIP samples to the arrays produced reproducible binding pat ...[more]
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