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Comparison of gene expression in wild-type Drosophila testes with tbrd-1 mutant testes

ABSTRACT: Gene expression is tightly linked to histone acetylation on lysine residues that can be recognized by bromodomains. The testis-specific bromodomain protein tBRD-1 is essential for male fertility and might act as a co-factor of testis-specifc TAFs. Here, we perform microarray analyses and demonstrate that tBRD-1 selectively controls gene expression in male germ cells Testes were dissected from 0-2 day old Drosophila males. The wild-type control sample was w1118. The tbrd-1 mutant is described in Leser et al., 2012 (Leser K, Awe S, Barckmann B, Renkawitz-Pohl R, Rathke C. 2012. The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertility. Biology Open 1: 597-606)

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Marek Bartkuhn

PROVIDER: E-GEOD-52511 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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tBRD-1 selectively controls gene activity in the Drosophila testis and interacts with two new members of the bromodomain and extra-terminal (BET) family.

Theofel Ina I Bartkuhn Marek M Hundertmark Tim T Boettger Thomas T Gärtner Stefanie M K SM Leser Katja K Awe Stephan S Schipper Michael M Renkawitz-Pohl Renate R Rathke Christina C

PloS one 20140924 9

Multicellular organisms have evolved specialized mechanisms to control transcription in a spatial and temporal manner. Gene activation is tightly linked to histone acetylation on lysine residues that can be recognized by bromodomains. Previously, the testis-specifically expressed bromodomain protein tBRD-1 was identified in Drosophila. Expression of tBRD-1 is restricted to highly transcriptionally active primary spermatocytes. tBRD-1 is essential for male fertility and proposed to act as a co-fa ...[more]

PMID: 25251222

Similar Datasets

Comparison of gene expression in wild-type Drosophila testes with tbrd-1 mutant testes

Project description:Gene expression is tightly linked to histone acetylation on lysine residues that can be recognized by bromodomains. The testis-specific bromodomain protein tBRD-1 is essential for male fertility and might act as a co-factor of testis-specifc TAFs. Here, we perform microarray analyses and demonstrate that tBRD-1 selectively controls gene expression in male germ cells

2014-08-08 | GSE52511 | GEO

Comparison of gene expression in control bam-Gal4 and control undriven tbrd-2RNAi Drosophila testes with tbrd-2 knockdown testes

Project description:Gene expression is tightly linked to histone acetylation on lysine residues that can be recognized by bromodomains. The testis-specific bromodomain protein tBRD-2 is essential for male fertility, interacts with tBRD-1 and might act as a co-factor of testis-specifc TAFs. Here, we perform microarray analyses and demonstrate that tBRD-2 selectively controls gene expression in male germ cells.

2017-03-23 | GSE81019 | GEO

Integrative analysis of phenotype and testicular quantitative proteome of CKLF-like MARVEL transmembrane domain-containing protein 4 (CMTM4) deficiency mouse reveal its association with male fertility

Project description:iTRAQ quantitative proteome of wild and CMTM4 knockout mouse testis tissue.Integrative analysis of phenotype and testicular quantitative proteome of CKLF-like MARVEL transmembrane domain-containing protein 4 (CMTM4) deficiency mouse reveal its association with male fertility.

2019-03-18 | PXD011936 | Pride

RNA-seq of Drosophila melanogaster testis with or without tbrd-1 knock out or tplus3a/b knock out

Project description:Many protein complexes are involved in gene regulation during Drosophila spermatogenesis. tplus3a and tplus3b code for Plus3 domain proteins and are enriched in spermatocytes. Male flies ∆tplus3a/b carrying deletions of both genes in trans to deficiency Df(2L)BSC151 show severely reduced fertility. tbrd-1 is known to regulate hundreds of genes during spermatogenesis in cooperation with tTAFs. To gain more insight into the regulatory mechanisms during spermatogenesis we used RNA from testes of ∆tplus3a/b/Df(2L)BSC151 and tbrd-11 mutant flies for RNAseq in comparison to w1118 wild-typic control. RNA was isolated with Trizol from 200 Testes dissected from w1118, homozygous tbrd-11 mutants or ∆tplus3a/b/Df(2L)BSC151. After DNAse I digestion RNA was purified and RNAseq was performed in three replicates. Genotype Description: w1118: wild-typic control tbrd-11: homozygous tbrd-11 mutants (knock-out through P{EPgy2}tbrd-11, as described in Leser et al., 2012) ∆tplus3a/b/Df(2L)BSC151: CRISPR/Cas9 deletion mutants, representing knock-out of both tplus3 and tplus3b. Male flies y1cho2v1;∆tplus3a/b/CyO were crossed with virgin females w1118;Df(2L)BSC151/CyO. Male offspring with w1118;∆tplus3a/b/Df(2L)BSC151 were used for RNAseq.

2019-11-04 | E-MTAB-7013 | biostudies-arrayexpress

Differential protein expression profiles of testes in Tmprss12-/- and wild-type mice

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2022-05-20 | PXD028014 | Pride

Expression data from P15 mouse testes control and Ikbkap-knockout mice

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2013-12-31 | E-GEOD-42230 | biostudies-arrayexpress

mRNA-Seq profiling of Stra8-deficient and wild-type testes

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2015-04-03 | E-GEOD-67169 | biostudies-arrayexpress

Transcription profiling of mouse WT and Nxf2 KO post-natal day 21 testes

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2009-11-14 | E-GEOD-13526 | biostudies-arrayexpress

Hipk4 is a manchette-associated protein essential for spermiogenesis

Project description:Nonobstructive azoospermia (NOA) is one of the most severe forms of male infertility, characterized by the absence of spermatozoa in the ejaculate. The etiology and genetic mechanism(s) of this disease remain largely unclear. Using Sanger sequence, we identified 12 heterozygous missense mutations and 2 heterozygous nonsense mutations in the HIPK4, encoding a serine/threonine kinase. Moreover, K490* mutant cause HIPK4 unstable in 293T cells. In accord with these findings, we observed that homozygous Hipk4-/- male mice were infertility owing to malformed and immotile spermatozoa, which are characterized by a deformed nucleus and a detached acrosome. Furthermore, we performed a phosphoproteomic analysis of testis from Hipk4 wild type and knockout mice, revealing multiple targets regulated by Hipk4 during spermiogenesis. Notably, manchette-associated protein Rimbp3 has multiple phosphorylation sites regulated by Hipk4. Moreover, Hipk4 and Rimbp3 deficiency mice display several common phenotypes, in particular with regard to the ectopic positioning of the manchette within spermatids, a presumed cause of sperm head deformities. Thus, Hipk4 might regulate manchette function through phosphorylation of Rimbp3, thereby playing essential role for sperm head formation and male fertility.

2022-08-02 | PXD019303 | Pride

Transcription profiling of mouse testis at developmental time points

Project description:The overall objective of this proposal is to map the temporal and spatial dynamics of gene expression in the fetal mouse testis at key developmental timepoints. Urogenital tract malformations are the most common birth defects in males and their incidence together with other male reproductive health concerns such as reduced fertility and testicular cancer are reportedly on the rise in the human population. To better understand the impact of genetic factors and environmental influences on testicular development, it is important to first understand normal gene expression patterns and signaling cascades within the fetal testis during development. The goal of this study is to identify cell-specific genes that can be used as biomarkers for key differentiation events.

2007-12-20 | E-GEOD-4818 | biostudies-arrayexpress

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