Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Integrative analysis of phenotype and testicular quantitative proteome of CKLF-like MARVEL transmembrane domain-containing protein 4 (CMTM4) deficiency mouse reveal its association with male fertility

ABSTRACT: iTRAQ quantitative proteome of wild and CMTM4 knockout mouse testis tissue.Integrative analysis of phenotype and testicular quantitative proteome of CKLF-like MARVEL transmembrane domain-containing protein 4 (CMTM4) deficiency mouse reveal its association with male fertility.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis, Epithelial Cell

DISEASE(S): Disease Free

SUBMITTER: Mingchao Wang

LAB HEAD: wang mingchao

PROVIDER: PXD011936 | Pride | 2019-03-18

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20171016_LL11-1.mzML	Mzml
	20171016_LL11.mzid.gz	Mzid
	20171016_LL11.raw	Raw
	20171016_LL12.mzML	Mzml
	20171016_LL12.raw	Raw

Items per page:

1 - 5 of 17

Publications

Integrated Analyses of Phenotype and Quantitative Proteome of CMTM4 Deficient Mice Reveal Its Association with Male Fertility.

Liu FuJun F Liu XueXia X Liu Xin X Li Ting T Zhu Peng P Liu ZhengYang Z Xue Hui H Wang WenJuan W Yang XiuLan X Liu Juan J Han WenLing W

Molecular & cellular proteomics : MCP 20190313 6

The chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing family (CMTM) is a gene family that has been implicated in male reproduction. CMTM4 is an evolutionarily conserved member that is highly expressed in the testis. However, its function in male fertility remains unknown. Here, we demonstrate that CMTM4 is associated with spermatogenesis and sperm quality. Using Western blotting and immunohistochemical analyses, we found CMTM4 expression to be decreased in poor-quality hum ...[more]

PMID: 30867229

Similar Datasets

Sperm proteome of CMTM4-deficient mice

Project description:Cmtm4 Knockout mouse was constructed by CRISPR-Cas9 technology, and the KO mice showed male infertility. A proteomic anlaysis of sperm proteins was performed by comparing KO and WT mice.

2019-05-21 | PXD013092 | Pride

Wdr13-/0 compared to wild-type mice hippocampus and pre-frontal cortex iTRAQ proteomics

Project description:Wdr13 has been implicated in memory and mental disorders, particularly in X-linked intellectual disability (XLID) in animal studies and in humans. The exact molecular function of Wdr13 is still largely unknown. In order to find out the role of Wdr13 in brain, we chose to characterize Wdr13 gene knockout mice and dissect the molecular mechanisms behind its action. We found that Wdr13 expresses majorly in hippocampal formation, cerebral cortex, cerebellum and striatum. Wdr13-/0 mice show mild anxiety but fared better than the wild type mice in memory tests. This phenotype was found to be correlated with increase in level of synaptic genes like Syn1, Camk2a, Sv2b and voltage gated ion channels. Interestingly, exposure to three/four weeks of social isolation caused symptoms of major depressive disorder in the Wdr13-/0 mice which was associated with loss in dendritic branches in hippocampal CA1 neurons and significant decrease in synaptic genes like Syn1, Rab3a, Nrxn2. We found GATA1, a common negative Transcription Factor for the above-mentioned and also a marker of MDD (Major Depressive Disorder) to be upregulated in the Wdr13-/0 mice after social isolation. WDR13 overexpression caused decrease in GATA1 expression in IMR32- human neuroblastoma cell line indicating a possible regulation of GATA1 by WDR13. Wdr13 thus becomes an important candidate gene to be involved in Major Depression.

2017-01-03 | PXD002466 | Pride

Analysis of protein expression in the lung of mice infected with influenza virus(WSN)

Project description:In order to analysis the changes of protein expression and its influence on innate immune in mice lung after influenza virus infection,we detect protein expression profile by iTRAQ method

2020-07-06 | PXD017061 | Pride

iTRAQ Analysis of DJ-1 Knockout Rodent Brains

Project description:Mutations in the DJ-1 (Park7) gene cause autosomal recessive Parkinson's disease in humans, but the function of the DJ-1 protein is poorly characterized. In an effort to understand more about the biology of DJ-1, we performed iTRAQ analyses on subcellular fractions enriched from DJ-1 knockout rat and mouse brains. We generated iTRAQ datasets for mitochondria and cytosol enriched fractions from 6-month-old rat brains, and the cytosol enriched fraction from 14-15-month old mouse brains. Our subsequent analyses of these datasets led to our discovery that the Hexokinase 1 protein was increased in the cytosol components of both DJ-1 knockout species.

2017-10-04 | PXD005215 | Pride

wdr13 knockout mice develops MD like phenotype on chronic stress

Project description:WDR13 expresses from the X chromosome and has a highly conserved coding sequence. There have been multiple associations of WDR13 with memory and psychiatric disorders. However, its detailed function in context of brain and behavior remains unknown. We characterized the behavioral phenotype of male mice lacking the homologue of WDR13 gene (Wdr13-/0). Mice were evaluated for changes in anxiety, learning and memory, and chronic stress induced behavioral deficits. Taking cue from analysis of its expression in the brain, we chose hippocampus and pre-frontal cortex for molecular studies to delineate its function. In the absence of Wdr13, there was a significant upregulation of synaptic proteins, viz., SYN1, RAB3A, CAMK2A etc accompanied with better memory retention in mice. Interestingly, three weeks social isolation stress resulted in downregulation of aforementioned synaptic proteins, decreased exploration time in central area of Open field test, increased immobility in Forced swim test, and reduced dendritic arborations of hippocampal CA1 neurons- hallmarks of Major Depressive Disorder (MDD) in Wdr13-/0 mice. This phenotype could be rescued by short imipramine treatment. We hypothesise that one of the key factors behind the MDD like phenotype in these mice was upregulation of transcription factor GATA1. Parallel work from our lab has indicated WDR13 to interact with multiple nuclear receptors and acting majorly as a co-repressor. Absence of Wdr13 resulted in de-regulated expression of a number of genes including multiple synaptic genes leading to observed phenotypes. Summarily, our data provides functional evidence for the role of Wdr13 in memory retention and in withstanding chronic stress induced depression.

2018-05-28 | PXD004545 | Pride

the Interaction Proteome of Ribosomal 40S Components PNO1 and NOB1 Using TurboID Proximity Labeling Technology

Project description:Background: The ribosome assembly factors PNO1 and NOB1 play crucial roles in the maturation of the 40S ribosomal small subunit. TurboID is an efficient biotin ligase that can biotinylate proteins in proximity to the target protein and is widely used to study complex biological processes within cells.Here, we utilized this technology to investigate the complex interaction network of PNO1 and NOB1 within cells.

2024-06-08 | PXD052825 | Pride

Hipk4 is a manchette-associated protein essential for spermiogenesis

Project description:Nonobstructive azoospermia (NOA) is one of the most severe forms of male infertility, characterized by the absence of spermatozoa in the ejaculate. The etiology and genetic mechanism(s) of this disease remain largely unclear. Using Sanger sequence, we identified 12 heterozygous missense mutations and 2 heterozygous nonsense mutations in the HIPK4, encoding a serine/threonine kinase. Moreover, K490* mutant cause HIPK4 unstable in 293T cells. In accord with these findings, we observed that homozygous Hipk4-/- male mice were infertility owing to malformed and immotile spermatozoa, which are characterized by a deformed nucleus and a detached acrosome. Furthermore, we performed a phosphoproteomic analysis of testis from Hipk4 wild type and knockout mice, revealing multiple targets regulated by Hipk4 during spermiogenesis. Notably, manchette-associated protein Rimbp3 has multiple phosphorylation sites regulated by Hipk4. Moreover, Hipk4 and Rimbp3 deficiency mice display several common phenotypes, in particular with regard to the ectopic positioning of the manchette within spermatids, a presumed cause of sperm head deformities. Thus, Hipk4 might regulate manchette function through phosphorylation of Rimbp3, thereby playing essential role for sperm head formation and male fertility.

2022-08-02 | PXD019303 | Pride

Large conserved domains of low DNA methylation maintained by 5-hydroxymethycytosine and Dnmt3a (CMS bisulfite-seq)

Project description:Gains and losses in DNA methylation are prominent genomic features of all mammalian cell types. To gain insight into mechanisms that could promote shifts in DNA 5-hydroxymethylation (5hmc) patterns and thus contribute to cell fate, including malignant transformation, we performed genome-wide mapping of 5hmc in purified wild type and Dnmt3a conditional knockout hematopoietic stem cells (HSCs) using cytosine-5-methylenesulphonate sequencing (CMS-seq). Comparing anti-CMS pulldown to input control, we identified 107,549 and 175,682 peaks of 5hmc enrichment in control and Dnmt3a knockout HSCs, respectively. Whole genome CMS-enriched bisulfite sequencing of secondarily-transplanted wild-type and Dnmt3a conditional knockout hematopoietic stem cells using Illumina HiSeq 2000

2014-01-22 | E-GEOD-53742 | biostudies-arrayexpress

Overexpression of fatty acid amide hydrolase induces early flowering in Arabidopsis thaliana

Project description:To better understand the molecular basis for the early flowering phenotype of AtFAAH overexpressors, microarray analysis was conducted comparing transcript profiles of wild type to the AtFAAH overexpressors and AtFAAH knockouts.

2012-02-01 | E-MEXP-3486 | biostudies-arrayexpress

Changes in proteome upon deletion of UBE2S/UBE2C in HCT116 cells

Project description:The proteome of HCT116 cells deleted of UBE2S and/or UBE2C was compared to the proteome of wild-type HCT116 cells.

2022-02-28 | PXD003443 | Pride