Proteomics

Dataset Information

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Differential protein expression profiles of testes in Tmprss12-/- and wild-type mice


ABSTRACT: Serine protease is considered a relative upstream regulator in the physiological processes including male reproduction. Transmembrane serine protease 12 (TMPRSS12) has been shown to regulate sperm motility and uterotubal junction migration in mice, but its role in the testis remains unknown.In this study, we confirmed the important role of TMPRSS12 in spermatogenesis. To further investigate the mechanism of TMPRSS12 in male reproduction, we used 2-D electrophoresis combined with Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry(MALDI-TOF/TOF) to construct differential protein expression profiles of testes in Tmprss12-/- and WT mice. A total of 64 spots with 2-fold or more differential expression between two groups were identified using MALDI-TOF/TOF. As a result, 54 unique proteins were identified successfully, including 39 proteins with decreased expression and 15 proteins with increased expression in Tmprss12-/- mice.

INSTRUMENT(S): ultraflex

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: Xinli Zhou  

LAB HEAD: Hui Zhu

PROVIDER: PXD028014 | Pride | 2022-05-20

REPOSITORIES: Pride

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Publications

TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice.

Zhang Jingjing J   Zhou Xinli X   Wan Danyang D   Yu Li L   Chen Xu X   Yan Tong T   Wu Zhu Z   Zheng Meimei M   Zhu Feng F   Zhu Hui H  

Frontiers in cell and developmental biology 20220425


Serine proteases are involved in many physiological activities as initiators of proteolytic cascades, and some members have been reported to play roles in male reproduction. Transmembrane serine protease 12 (TMPRSS12) has been shown to regulate sperm motility and uterotubal junction migration in mice, but its role in the testis remains unknown. In this study, we verified that TMPRSS12 was expressed in the spermatocytes and spermatids of testis and the acrosome of sperm. Mice deficient in <i>Tmpr  ...[more]

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