Unknown,Transcriptomics,Genomics,Proteomics

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Deciphering the genetic basis of Moraxella catarrhalis complement resistance: Gene expression analysis of Moraxella catarrhalis BBH18 upon exposure to normal human serum.


ABSTRACT: The complement system is an important part of the innate defense against invading pathogens. The ability to resist complement-mediated killing is considered to be an important virulence trait for the human-restricted respiratory tract pathogen M. catarrhalis, as most disease-associated M. catarrhalis isolates are complement-resistant. Here we studied the molecular basis of M. catarrhalis complement-resistance by transcriptome profiling upon exposure to 10% normal human serum (NHS). After 1h exposure of M. catarrhalis BBH18 to 10% NHS (n = 5) or the NHS dilution buffer alone (control, n = 5), total RNA was isolated and labeled cDNA was generated according to standard Nimblegen gene expression array protocols and hybridized to 4x72K Nimblegen M. catarrhalis expression arrays for read-out.

ORGANISM(S): Moraxella catarrhalis BBH18

SUBMITTER: Hester Bootsma 

PROVIDER: E-GEOD-52552 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Deciphering the genetic basis of Moraxella catarrhalis complement resistance: a critical role for the disulphide bond formation system.

de Vries Stefan P W SP   Rademakers Rob J A RJ   van der Gaast-de Jongh Christa E CE   Eleveld Marc J MJ   Hermans Peter W M PW   Bootsma Hester J HJ  

Molecular microbiology 20131218 3


The complement system is an important innate defence mechanism, and the ability to resist complement-mediated killing is considered a key virulence trait of the respiratory tract pathogen M. catarrhalis. We studied the molecular basis of complement resistance by transcriptional profiling and Tn-seq, a genome-wide negative-selection screenings technology. Exposure of M. catarrhalis to human serum resulted in increased expression of 84 genes and reduced expression of 134 genes, among which genes e  ...[more]

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