Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

30 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Real-time quantitative PCR analysis of mice lung tissue

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Kenneth Hsu

PROVIDER: E-GEOD-52720 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

S100A8 induces IL-10 and protects against acute lung injury.

Hiroshima Yuka Y Hsu Kenneth K Tedla Nicodemus N Chung Yuen Ming YM Chow Sharron S Herbert Cristan C Geczy Carolyn L CL

Journal of immunology (Baltimore, Md. : 1950) 20140214 6

S100A8 is considered proinflammatory by activating TLR4 and/or the receptor for advanced glycation end products. The aim was to investigate inflammatory effects of S100A8 in murine lung. S100A8 was administered to BALB/c mice by nasal inhalation and genes induced over a time-course assessed. LPS was introduced intranasally either alone or 2 h after pretreatment of mice with intranasal application of S100A8 or dexamethasone. A Cys(42)-Ala(42) mutant S100A8 mutant was used to assess whether S100A8 ...[more]

PMID: 24532576

Similar Datasets

Real-time quantitative PCR analysis of mice lung tissue (1)

Project description:Recombinant-murine S100A8 and the corresponding Cys42 to Ala42 mutant were used at 10 mg/50 ml HBSS administered onto the nares of BALB/C mice. To assess direct effects, mice were sacrificed 1, 4, 6, 12 or 20 h post-inhalation of S100A8 or 12 and 20 h post-inhalation of Ala42S100A8. For comparison with Dex inhalation (used at 10 mg/50 ml HBSS), lungs were harvested 6 and 12 h post administration. Because S100A8 is reported to initiate proinflammatory responses by ligating TLR4 and/or RAGE, a quantitative PCR array was developed to analyse 63 genes, selected to reflect potential acute inflammatory changes induced by ligation of these receptors. Expression of inflammatory genes was evaluated with the RT-qPCR array. Relative quantities of mRNA in duplicate samples were obtained using the LightCyclerM-BM-. 480 Software 1.5 and the Efficiency-Method.

2013-11-26 | E-GEOD-52718 | biostudies-arrayexpress

Real-time quantitative PCR analysis of mice lung tissue (2)

2013-11-26 | E-GEOD-52719 | biostudies-arrayexpress

Microbe-induced inflammatory cytokines in Il10-/- colon tissue

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2012-07-04 | E-GEOD-39085 | biostudies-arrayexpress

Colon tissue of Il10-/- mice monoassociated with Escherichia coli NC101 or Escherichia faecalis OG1RF (PAMM-077 array)

Project description:We observed that interleukin-10 knockout (Il10-/-) mice injected with azoxymethane (AOM) and monoassociated with E. coli NC101, but not E. faecalis OG1RF, develop tumors. Histologic inflammation is not different in mice monoassociated with either bacterium. The goal of this experiment is to assess inflammatory cytokine levels in colonic tissue of these mice. 4 germ-free Il10-/- mice were assayed and used as controls. 4 E. coli and 4 E. faecalis monoassociated mice were experimental samples.

2012-07-04 | E-GEOD-39092 | biostudies-arrayexpress

Colon tissue of Il10-/- mice monoassociated with Escherichia coli NC101 or NC101-delta-pks

Project description:We observed that deletion of polyketide synthase (pks) from E. coli NC101 reduces its ability to induce tumors in interleukin-10 knockout (Il10-/-) mice injected with azoxymethane (AOM), without altering histologic inflammation. The goal of this experiment is to assess inflammatory cytokine levels in colonic tissue of these mice. 2 germ-free Il10-/- mice were assayed and used as controls. 3 E. coli NC101 and 3 E. coli NC101-delta-pks monoassociated mice were experimental samples.

2012-07-04 | E-GEOD-39095 | biostudies-arrayexpress

Colon tissue of Il10-/- mice monoassociated with Escherichia coli NC101 or Escherichia faecalis OG1RF (PAMM-073 array)

2012-07-04 | E-GEOD-39094 | biostudies-arrayexpress

PCR array analysis of DNA repair gene expression in human vascular smooth muscle cells

Project description:cDNA from aortic and plaque VSMCs was isolated and subject to PCR array analysis VSMCs were cultured from human tissue samples and used at passage number 5 or below. qPCR gene expression profiling. VSMCs from 9 donors ( 5 disease free aortic tissue as controls and 4 diseased carotid plaques). Equal amounts of RNA was used from each patient sample. RNA was not pooled.

2014-09-01 | E-GEOD-59030 | biostudies-arrayexpress

Real-time quantitative PCR analysis of mouse lungs

Project description:Balb/cJ mouse received control RNAi or RNAi to 8-oxoguanine DNA glycosylase (Ogg1) intranasally prior to the exposure for 1 h to a) glucose oxidase (1 mU) to induce OGG1-BER or b) OGG1-BER product 8-oxoguanine (1 M-NM-<M). We used SABiosciences Mouse Inflammatory Cytokines & Receptors PCR Array (PAMM-011A) to quantitate inflammatory gene expression dependent on OGG1 and its product 8-oxoguanine. After intranasal challenge with glucose oxidase or 8-oxoguanine, mice were sacrificed and lungs were collected and processed to obtain RNA. Total pooled (n=5) RNA (1 M-NM-<g) was reverse transcribed into cDNA using SuperscriptM-BM-. III First Strand Synthesis System (Invitrogen), mixed with equal amounts of 2X SYBR Green Supermix (Qiagen) and 20 M-NM-<l of reaction mixture was added to each well of the PAM-011A array. The reaction was evaluated using an ABI PRISMM-BM-. 7000 Sequence Detection System using recommended settings by SABiosciences.

2014-08-26 | E-GEOD-60711 | biostudies-arrayexpress

Gene expression analysis of eight-cell stage rat embryos sired by paternally exposed males to saline or BEP

Project description:8-cell stage rat embryos were sired by males treated with saline or a chemotherapeutic cocktail, BEP, consisting of Bleomycin, Etoposide, and Cisplatin for 9 weeks. Animals were given an additional 9 weeks of recovery, without any treatment, prior to mating. qPCR gene expression profiling for 84 stem cell transcription factors. Total RNA extracted from 25-35 embryos at the eight-cell stage sired by control or BEP-treated males after a 9-wk recovery period (2 females mated per male, n= 4 males/treatment).

2014-03-13 | E-GEOD-55842 | biostudies-arrayexpress

Changes in gene expression analysis of alphaCD3alpha/CD28 activated human naive and memory regulatory T cells (Treg) upon IL-7 treatment as measured by quantitative PCR array

Project description:This study evaluated changes in gene expression upon IL-7 treatment in human naive and memory Treg. CD4+CD25+CD127low naïve and memory Treg were isolated from fresh PBMC and separately stimulated with ?CD3?/CD28 coupled beads (Invitrogen-Dynal) at a 1:10 bead/T cell ratio, treated with or without 10 ng/ml of rhIL-7 (R&D Systems) for 16 hours. qPCR gene expression profiling of CD4+CD25+CD127low naïve and memory Treg obtained from 2 separate donors. Cell lysates were prepared separately from the 2 donors and pooled prior to RNA extraction.

2012-09-12 | E-GEOD-40755 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data