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Gene expression in 2,3-Pentanedione Induced Bronchiolitis Oblliterans

ABSTRACT: Fibrotic and nonfibrotic bronchial tissues were collected from lungs of pentandione exposed rats by laser capture microdissection. Analogous tissue was collected from air-exposed controls. Miroarray analysis revealed a number of DEGs Male Wistar Han rats were exposed to either air or 200 ppm 2,3-pentanedione for 2 wks. Fibrotic bronchial lesions were collected form exposed rats and analogous bronchial tissue was collected from controls. RNA was extracted and amplified to cDNA which was hybridized to Affymetrix microarrays to Rat Gene 1.0 ST Array. Transcript profiles from control and treated rats were compared.

ORGANISM(S): Rattus norvegicus

SUBMITTER: NIEHS Microarray Core

PROVIDER: E-GEOD-52761 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression in obliterative bronchiolitis-like lesions in 2,3-pentanedione-exposed rats.

Morgan Daniel L DL Merrick B Alex BA Gerrish Kevin E KE Stockton Patricia S PS Wang Yu Y Foley Julie F JF Gwinn William M WM Kelly Francine L FL Palmer Scott M SM Ton Thai-Vu T TV Flake Gordon P GP

PloS one 20150224 2

Obliterative bronchiolitis (OB) is an irreversible lung disease characterized by progressive fibrosis in the small airways with eventual occlusion of the airway lumens. OB is most commonly associated with lung transplant rejection; however, OB has also been diagnosed in workers exposed to artificial butter flavoring (ABF) vapors. Research has been limited by the lack of an adequate animal model of OB, and as a result the mechanism(s) is unclear and there are no effective treatments for this cond ...[more]

PMID: 25710175

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