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Analysis of the apical secretome of Calu-3 and NHBE cells in 3D models of the human bronchial epithelium

ABSTRACT: The mucus secreted by airway epithelial cells plays a key role in the protection against and clearance of particles and pathogens. In this project, a 3D model of the bronchial epithelium was established using Calu-3 cells grown on porous inserts at the air-liquid interface. The secreted proteins were characterized in long-term cultures and compared to the apical secretome of primary normal human bronchial epithelial (NHBE) cells. The apical secretome was collected and characterized in the Calu-3 model at day 4, day 11, and day 18 after air-liquid interface and in the NHBE model at day 4, day 12, and day 18 after air-liquid interface. "This project received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement no 760928 (BIORIMA)."

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Epithelial Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: Daniel Sanchez-Guzman

LAB HEAD: Armelle Baeza Squiban

PROVIDER: PXD024242 | Pride | 2021-03-26

REPOSITORIES: Pride

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			Action	DRS
	1929001-Q11.raw	Raw
	1929001-Q15.raw	Raw
	1929001-Q16.raw	Raw
	1929001-Q17.raw	Raw
	1929001-Q18.raw	Raw

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miRNA expression in differentiating primary human bronchial epithelial cells cultured at air-liquid-interface

Project description:We performed microarray analysis of miRNA expression in differentiating primary human bronchial epithelial cells. The goal was to identify miRNAs that are dynamically expressed under airway epithelial development. Cells were cultured at air-liquid-interface and were harvested day 4, 6, 8, 11, 13, 15, 18, 20 and 22 for analysis.

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