Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of p68/72 deficient C2C12 cells cultured in either growth medium or differentiation medium to investigate the function of p68/p72 in muscle gene expression and skeletal C2C12 cell differentiation.

ABSTRACT: This project investigates the function of p68/p72 in muscle gene expression and skeletal C2C12 cell differentiation. p68/p72 in C2C12 cells cultured in either growth medium (GM) or differentiation medium (DM) was knocked down using shRNA p68/p72 retrovirus, and globle gene expression was profiled using Affymetrix MOE 430A and 430B GeneChip. Experiment Overall Design: The specific aim is to study muscle gene expression changes and skeletal muscle differenciation using RNA interference of p68/p72 in C2C12 cells.

ORGANISM(S): Mus musculus

SUBMITTER: Eric Hoffman

PROVIDER: E-GEOD-5305 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

p68 SMC differentiation

Project description:This project investigates the function of p68/p72 in muscle gene expression and skeletal C2C12 cell differentiation. p68/p72 in C2C12 cells cultured in either growth medium (GM) or differentiation medium (DM) was knocked down using shRNA p68/p72 retrovirus, and globle gene expression was profiled using Affymetrix MOE 430A and 430B GeneChip. Keywords: Time-Series

2006-07-13 | GSE5305 | GEO

C2C12 Mouse Skeletal Muscle Cells Grown in Differentiation Medium for 5 Days.

Project description:C2C12 mouse skeletal muscle cells grown in differentiation medium for 5 days. Keywords: other

2005-12-09 | GSE3787 | GEO

Transcription profiling of mouse C2C12 skeletal muscle cells grown in differentiation medium for 5 days

Project description:C2C12 mouse skeletal muscle cells grown in differentiation medium for 5 days. Experiment Overall Design: this experiment include 1 samples and 9 replicates

2008-06-12 | E-GEOD-3787 | biostudies-arrayexpress

Comprehensive analysis of the proteome, phosphoproteome and N-linked sialylated glycoproteome of C2C12 myoblasts

Project description:C2C12 myoblast is a model that has been used extensively to study the process of skeletal muscle differentiation. Proteomics has advanced our understanding of skeletal muscle biology and the process of myogenesis. However, there is still no deep coverage of C2C12 myoblast proteome, which is important for the understanding of key drivers for the differentiation of skeletal muscle cells. Here, we conducted a multi-dimensional proteome profiling with TiSH strategy to get a comprehensive analysis of proteome, phosphoproteome and N-linked sialylated glycoproteome of C2C12 myoblasts. A total of 8313 protein groups were identified in C2C12 myoblasts, including 7827 protein groups from non-modified peptides, 3803 phosphoproteins and 977 formerly N-linked sialylated glycoproteins.

2020-11-27 | PXD011334 | Pride

FoxO RNAi in C2C12 cells

Project description:C2C12 cells are mouse skeletal muscle cells. These cells were transfected with shRNA against FoxO1, FoxO3, and FoxO4. FoxO1, FoxO3, and FoxO4 are the known paralogues expressed in this cell line.

2008-12-31 | GSE13347 | GEO

Differentially expressed genes in c2c12 myoblasts

Project description:Proteosome inhibitors such as bortezomib (BTZ) have been used to treat muscle wasting in animal models. However, direct effect of BTZ on skeletal muscle cells has not been reported. In the present study, our data showed that C2C12 cells exhibited a dose-dependent decrease in cell viability in response to increasing concentrations of BTZ. We used microarrays to detail the differentially expressed genes in c2c12 myoblasts with different culture conditions.

2016-12-23 | GSE92671 | GEO

Deep muscle proteome - Deep skeletal muscle proteome. Triceps muscle from C57BL6 mouse and C2C12 myotubes were measure by LC-MS.

Project description:Deep skeletal muscle proteome. Triceps muscle from C57BL6 mouse and C2C12 myotubes were measure by LC-MS.

2015-04-08 | PXD000288 | Pride

E-GEOD-5305

Project description:Transcription profiling of p68/72 deficient C2C12 cells to investigate the function of p68/p72 in muscle gene expression and skeletal C2C12 cell differentiation.

2015-10-13 | E-GEOD-5305 | ExpressionAtlas

Effect of ATF4 overexpression on mRNA levels in differentiated C2C12 myotubes.

Project description:ATF4 is a bZIP transcription factor that that promotes skeletal muscle atrophy. The goal of these studies was to determine the effects of ATF4 overexpression on mRNA levels in differentiated C2C12 myotubes. For additional details see Ebert et al, Stress-Induced Skeletal Muscle Gadd45a Expression Reprograms Myonuclei and Causes Muscle Atrophy. JBC epub. June 12,2012 C2C12 myotubes were infected with adenovirus co-expressing eGFP and ATF4-FLAG. Control myotubes were infected with adenovirus co-expressing eGFP and a transcriptionally inactive ATF4 construct (ATF4∆bZIP).

2012-09-15 | E-GEOD-39194 | biostudies-arrayexpress

Effect of ATF4 overexpression on mRNA levels in differentiated C2C12 myotubes.

2012-09-15 | GSE39194 | GEO