Dataset Information

Comprehensive analysis of the proteome, phosphoproteome and N-linked sialylated glycoproteome of C2C12 myoblasts

ABSTRACT: C2C12 myoblast is a model that has been used extensively to study the process of skeletal muscle differentiation. Proteomics has advanced our understanding of skeletal muscle biology and the process of myogenesis. However, there is still no deep coverage of C2C12 myoblast proteome, which is important for the understanding of key drivers for the differentiation of skeletal muscle cells. Here, we conducted a multi-dimensional proteome profiling with TiSH strategy to get a comprehensive analysis of proteome, phosphoproteome and N-linked sialylated glycoproteome of C2C12 myoblasts. A total of 8313 protein groups were identified in C2C12 myoblasts, including 7827 protein groups from non-modified peptides, 3803 phosphoproteins and 977 formerly N-linked sialylated glycoproteins.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Myoblast, Cell Culture

SUBMITTER: Xiulan Chen

LAB HEAD: Professor Fuquan Yang

PROVIDER: PXD011334 | Pride | 2020-11-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	C2C12deglycosylatedpeptides_replicate1_1.msf	Msf
	C2C12deglycosylatedpeptides_replicate1_1.raw	Raw
	C2C12deglycosylatedpeptides_replicate1_10.msf	Msf
	C2C12deglycosylatedpeptides_replicate1_10.raw	Raw
	C2C12deglycosylatedpeptides_replicate1_11.msf	Msf

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Publications

Comprehensive Analysis of the Proteome and PTMomes of C2C12 Myoblasts Reveals that Sialylation Plays a Role in the Differentiation of Skeletal Muscle Cells.

Chen Xiulan X Sun Yaping Y Zhang Tingting T Roepstorff Peter P Yang Fuquan F

Journal of proteome research 20201120 1

The C2C12 myoblast is a model that has been used extensively to study the process of skeletal muscle differentiation. Proteomics has advanced our understanding of skeletal muscle biology and also the differentiation process of skeletal muscle cells. However, there is still no comprehensive analysis of C2C12 myoblast proteomes, which is important for the understanding of key drivers for the differentiation of skeletal muscle cells. Here, we conducted multidimensional proteome profiling to get a c ...[more]

PMID: 33216553

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