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Deep sequencing of the murine olfactory receptor neuron transcriptome

ABSTRACT: The recent development of next-generation sequencing (NGS) techniques encouraged us to assess the transcriptome of the murine OE. We analyzed RNA from OEs of female and male adult mice and from fluorescence-activated cell sorting (FACS)-sorted olfactory receptor neurons (ORNs) obtained from transgenic OMP-GFP mice The Illumina RNA-Seq protocol was utilized to generate up to 86 million reads per transcriptome.

ORGANISM(S): Mus musculus

SUBMITTER: Ninthujah Kanageswaran

PROVIDER: E-GEOD-53793 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deep sequencing of the murine olfactory receptor neuron transcriptome.

Kanageswaran Ninthujah N Demond Marilen M Nagel Maximilian M Schreiner Benjamin S P BS Baumgart Sabrina S Scholz Paul P Altmüller Janine J Becker Christian C Doerner Julia F JF Conrad Heike H Oberland Sonja S Wetzel Christian H CH Neuhaus Eva M EM Hatt Hanns H Gisselmann Günter G

PloS one 20150115 1

The ability of animals to sense and differentiate among thousands of odorants relies on a large set of olfactory receptors (OR) and a multitude of accessory proteins within the olfactory epithelium (OE). ORs and related signaling mechanisms have been the subject of intensive studies over the past years, but our knowledge regarding olfactory processing remains limited. The recent development of next generation sequencing (NGS) techniques encouraged us to assess the transcriptome of the murine OE. ...[more]

PMID: 25590618

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