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Developmental Dynamics of Kranz Cell Transcriptional Specificity in Maize Leaf Reveals Early Onset of C4-related Processes


ABSTRACT: The comparison of the cell-specific transcriptomes of bundle sheath (BS) and mesophyll (M) cells from successive developmental stages of maize leafs reveals that the number of genes preferentially transcribed in one cell type or the other varies considerably from the sink-source transition to mature photosynthetic stages. The number of differentially expressed (DE) genes is maximal at a stage well prior to full maturity, including those that encode key functions for C4 photosynthesis. The developmental dynamics of BS/M differential expression can be used to identify candidates for other C4-related functions and to simplify the identification of specific pathways members from otherwise complex gene families. The candidates for C4-related transcription factors identified with this developmental DE strategy overlap with those identified in studies using alternative strategies. Nine day old third leaves of maize sections, located at -1 cm, +4 cm and +9 cm (leaf tip), relative to the sink-source transition, were collected. BS and M cells were captured from each section. There are two duplications for each section and each cell types. A total of 12 libraries were constructed for RNA-seq.

ORGANISM(S): Zea mays

SUBMITTER: Timothy Nelson 

PROVIDER: E-GEOD-54272 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Developmental dynamics of Kranz cell transcriptional specificity in maize leaf reveals early onset of C4-related processes.

Tausta S Lori SL   Li Pinghua P   Si Yaqing Y   Gandotra Neeru N   Liu Peng P   Sun Qi Q   Brutnell Thomas P TP   Nelson Timothy T  

Journal of experimental botany 20140430 13


The comparison of the cell-specific transcriptomes of bundle sheath (BS) and mesophyll (M) cells from successive developmental stages of maize (Zea mays) leaves reveals that the number of genes preferentially transcribed in one cell type or the other varies considerably from the sink-source transition to mature photosynthetic stages. The number of differentially expressed (DE) genes is maximal at a stage well before full maturity, including those that encode key functions for C4 photosynthesis.  ...[more]

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