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Analysis of genes regulated by Akirin and Brahma upon IMD pathway activation in drosophila S2 cells

ABSTRACT: This micro-array helps establishing the function of Akirin, a nuclear protein with unknown domains, a putative interacting partner, in the transcriptional regulation of the targets of Relish, a NF-kB factor required to fight Gram(-) bacteria infection in Drosophila melanogaster. S2 cells were knocked down for Relish, Akirin and immune-challenged by Calcium-phosphate transient transfection of dsRNA and over-expressing PGRP-LC vector. Positively transfected cells were sorted by co-expressed Tomato and RNA was purified and analysed by a micro-array

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Nicolas MATT

PROVIDER: E-GEOD-54915 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Akirin specifies NF-κB selectivity of Drosophila innate immune response via chromatin remodeling.

Bonnay François F Nguyen Xuan-Hung XH Cohen-Berros Eva E Troxler Laurent L Batsche Eric E Camonis Jacques J Takeuchi Osamu O Reichhart Jean-Marc JM Matt Nicolas N

The EMBO journal 20140901 20

The network of NF-κB-dependent transcription that activates both pro- and anti-inflammatory genes in mammals is still unclear. As NF-κB factors are evolutionarily conserved, we used Drosophila to understand this network. The NF-κB transcription factor Relish activates effector gene expression following Gram-negative bacterial immune challenge. Here, we show, using a genome-wide approach, that the conserved nuclear protein Akirin is a NF-κB co-factor required for the activation of a subset of Rel ...[more]

PMID: 25180232

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