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Unknown,Transcriptomics,Genomics,Proteomics

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Temporal Response to seasonal and pandemic H1N1 infection in human DCs- Donor 1

ABSTRACT: An 8 hours timecourse was performed with human DCs infected either with A/California/7/2009 and A/Brevig Mission/1/1918 (pandemic) or A/New Caledonia/20/99 and A/Texas/36/91 seosonal. Human monocyte derived dendritic cells (DCs) were infected either with chicken egg grown A/California/7/2009, A/Brevig Mission/1/1918, A/New Caledonia/20/99or A/Texas/36/91. Samples were taken and fixed in RNA stabilizing regaent at 2, 2.7, 3.3, 4, 5, 6, 7 and 8 hours. Cells exposed to chicken egg alantoic fluid served as a control. Infections with A/California/7/2009, A/New Caledonia/20/99 or A/Texas/36/91 were carried out at a BSL2 enviroment. Infections with A/Brevig Mission/1/1918 were carried out in a BSL3 enviroment in parralel to the BSL2 infections with cells from the same donor and cell preparation.

ORGANISM(S): Homo sapiens

SUBMITTER: Hailong Meng

PROVIDER: E-GEOD-55276 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Temporal Response to seasonal and pandemic H1N1 infection in human DCs - Donor2

Project description:An 8 hours timecourse was performed with human DCs infected either with A/California/7/2009 and A/Brevig Mission/1/1918 (pandemic) or A/New Caledonia/20/99 and A/Texas/36/91 seosonal. Human monocyte derived dendritic cells (DCs) were infected either with chicken egg grown A/California/7/2009, A/Brevig Mission/1/1918, A/New Caledonia/20/99or A/Texas/36/91. Samples were taken and fixed in RNA stabilizing regaent at 2, 2.7, 3.3, 4, 5, 6, 7 and 8 hours. Cells exposed to chicken egg alantoic fluid served as a control. Infections with A/California/7/2009, A/New Caledonia/20/99 or A/Texas/36/91 were carried out at a BSL2 enviroment. Infections with A/Brevig Mission/1/1918 were carried out in a BSL3 enviroment in parralel to the BSL2 infections with cells from the same donor and cell preparation.

2015-08-20 | E-GEOD-55277 | biostudies-arrayexpress

Temporal Response to seasonal and pandemic H1N1 infection in human DCs- Donor 1

2015-08-20 | GSE55276 | GEO

Temporal Response to seasonal and pandemic H1N1 infection in human DCs - Donor2

2015-08-20 | GSE55277 | GEO

Acute host transcriptonal response to A/Texas/36/91 influenza and A/Texas/36/91 influenza containing the NS1 gene from r1918 in A549 cells

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Omics analysis of host responses during pandemic H1N1 influenza infection

Project description:Proteomics data from lungs and trachea of ferrets (Mustela putorius furo) infected intranasally with either influenza A/California/04/2009 (CA04) virus or influenza A/Brevig Mission/1/1918 (1918) virus. Sections of trachea and lung collected were at 1, 3 and 8 days post-infection.

2015-04-24 | MSV000079114 | MassIVE

Acute host transcriptonal response to r1918 influenza and r1918 influenza containing the NS1 gene from A/Texas/36/91 in A549 cells

Project description:The “Spanish influenza” of 1918 claimed an unprecedented number of lives, yet the determinants of virulence for this virus are still not fully understood. Here, we used functional genomics and an in vitro human lung epithelial cell infection model to define the global host transcriptional response to the eight-gene 1918 virus. To better understand the role of the 1918 virus NS1 gene, we also evaluated the host response to fully reconstructed 1918 and reassortant 1918 virus containing the NS1 gene from A/Texas/36/91 (a seasonal isolate of human influenza virus). A549 cells were infected with either recombinant 1918 influenza, a chimeric virus that contained seven genes from recombinant 1918 and the NS1 from A/Texas/36/91 (Tx/91), or mock. Cells were harvested for microarray analysis at 2, 6, and 24hr following infection.

2012-05-13 | E-GEOD-37951 | biostudies-arrayexpress

Acute host transcriptonal response to A/Texas/36/91 influenza and A/Texas/36/91 influenza containing the NS1 gene from r1918 in A549 cells

Project description:The M-bM-^@M-^\Spanish influenzaM-bM-^@M-^] of 1918 claimed an unprecedented number of lives, yet the determinants of virulence for this virus are still not fully understood. Here, we used functional genomics and an in vitro human lung epithelial cell infection model to define the global host transcriptional response to the eight-gene 1918 virus. To better understand the role of the 1918 virus NS1 gene, we evaluated the host response to A/Texas/36/91 (a seasonal isolate of human influenza virus) and a reassortant of A/Texas/36/91 containing the 1918 NS1 gene. A549 cells were infected at a MOI=2 with either A/Texas/36/91 (Tx/91) or A/Texas/36/91 (Tx/91) containing the NS1 gene from r1918 influenza. Virus was allowed to bind to cells for 1 h at 4M-BM-0C in serum-free infection medium supplemented with trypsin (1 g/ml). Mock-infected cells were treated with allantoic fluid instead of virus. Three replicate wells were used for each infection condition at each time point. Cells were harvested for array analysis at 2, 6, and 24hr post-infection. Cells from three individual cultures were pooled for each condition for microarray.

2012-05-13 | E-GEOD-37829 | biostudies-arrayexpress

Acute host transcriptonal response to r1918 influenza and r1918 influenza containing the NS1 gene from A/Texas/36/91 in A549 cells

2012-05-14 | GSE37951 | GEO

2009 pandemic H1N1 virus causes disease and upregulation of genes related to inflammatory and immune response, cell death, and lipid metabolism in pigs

Project description:This study used virological, histological, and global gene expression data to compare the virulence of two 2009 pH1N1 isolates from human (A/California/04/2009) and swine (A/swine/Alberta/25/2009) to that of a 1918-like classical swine influenza virus (A/swine/Iowa/1930) in a pig model of infection. The overall goal of this study was to characterize the clinical, histological, virological and global gene expression responses to three distinct influenza A isolates in an experimental pig model of influenza infection. We compared the pathogenesis of two pH1N1 viruses, one derived from a human patient (A/CA/04/09 [CA09]) and the other from swine (A/swine/Alberta/25/2009 [Alb09]), with that of the 1918-like classical swine influenza virus (A/swine/Iowa/1930 [IA30]) in the pig model. Both pH1N1 isolates induced clinical symptoms such as coughing, sneezing, decreased activity, fever, and labored breathing in challenged pigs, but IA30 virus did not cause any clinical symptoms except fever. Although both the pH1N1 viruses and the IA30 virus caused lung lesions, the pH1N1 viruses were shed from the nasal cavities of challenged pigs whereas the IA30 virus was not. Microarray was used to assess global gene expression in the lungs at 3 and 5 days post-infection.

2012-02-13 | GSE35738 | GEO

Fribourg2014 - Model of influenza A virus infection dynamics of viral antagonism and innate immune response.

Project description:This is an ordinary differential equation mathematical model investigating the early responses of human monocyte-derived dendritic cells to infection by two H1N1 influenza A viruses of different clinical outcomes: pandemic A/California/4/2009 and seasonal A/New Caledonia/20/1999.

2024-09-02 | BIOMD0000000889 | BioModels

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