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Gene expression profiling in allele replacement panel strains containing all combinations of four sporulation QTN in S. cerevisiae

ABSTRACT: We study the effect of four QTN in RME1, IME1 & RSF1 that are causative for variation in sporulation efficiency. We investigate the relationship between genotype, gene expression and phenotype and whether the amount of gene expression variation explained by the sporulation QTN is predictive of the amount of phenotypic variation explained by them. RNA-Seq analysis of 4 replicates each of 16 allele replacement panel strains containing all combinations of the four sporulation QTN after 2 hours in sporulation medium.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Barak Cohen

PROVIDER: E-GEOD-55409 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single nucleotide variants in transcription factors associate more tightly with phenotype than with gene expression.

Sudarsanam Priya P Cohen Barak A BA

PLoS genetics 20140501 5

Mapping the polymorphisms responsible for variation in gene expression, known as Expression Quantitative Trait Loci (eQTL), is a common strategy for investigating the molecular basis of disease. Despite numerous eQTL studies, the relationship between the explanatory power of variants on gene expression versus their power to explain ultimate phenotypes remains to be clarified. We addressed this question using four naturally occurring Quantitative Trait Nucleotides (QTN) in three transcription fac ...[more]

PMID: 24784239

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