Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human H295R Cells Treated with ANGII, Potassium, or Forskolin for 3 Hours


ABSTRACT: H295R human adrenocortical cells were cultured in H295R complete media containing DMEM:F12 (1:1) supplemented with 2% Ultroser G (Biosepra, Villeneuve-la-Garenne, France), ITS-Plus (Discovery Labware, Bedford, MA) and an antibiotic/antimycotic mixture (Invitrogen, Carlsbad, CA) in 175 cm2 flasks until subconfluente. Media was replaced with 40 ml fresh media containing different agents and cultured for 3 h. Treatments: Angiotensin II (100 nM), potassium (16 mM) and forskolin (10 uM). Total RNA extracted with RNAesy Midi Kit (Qiagen) with on-column DNase digestion Experiment Overall Design: Controls, n=2 Experiment Overall Design: Angiotensin=1 Experiment Overall Design: Potassium=1 Experiment Overall Design: Forskolin=1 Experiment Overall Design: Same biological sample used for GPL96 and GPL97

ORGANISM(S): Homo sapiens

SUBMITTER: Damian Gaston Romero 

PROVIDER: E-GEOD-5553 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Adrenal transcription regulatory genes modulated by angiotensin II and their role in steroidogenesis.

Romero Damian G DG   Rilli Silvia S   Plonczynski Maria W MW   Yanes Licy L LL   Zhou Ming Yi MY   Gomez-Sanchez Elise P EP   Gomez-Sanchez Celso E CE  

Physiological genomics 20070227 1


Transcription regulatory genes are crucial modulators of cell physiology and metabolism whose intracellular levels are tightly controlled to respond to extracellular stimuli. We studied transcription regulatory genes modulated by angiotensin II, one of the most important regulators of adrenal cortical cell function, and their role in adrenal steroidogenesis in H295R human adrenocortical cells. Angiotensin II-modulated transcription regulatory genes were identified with high-density oligonucleoti  ...[more]

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