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Next Generation Sequencing of Wild-Type FVB/NJ Mouse Cardiac Polyadenylated RNA

ABSTRACT: Purpose: Next-generation sequencing (NGS) provides for quantitation of RNA abundances and comparison of RNA abundances within tissues and cells in a manner not possible with previous microarray technologies. We have made widespread use of Illumina sequencing technologies for RNA quantitation in several publications involving mouse hearts, dating from 2010, and wish to share both high-quality raw sequencing data and data processed to quantitate mRNA abundances from wild-type mice, male and female, at a variety of ages. These data will provide a resource for investigators using microarrays to understand the concentration of transcripts of interest relative to other cardiac RNAs, and will permit deeper interpretation of previous microarray studies. 40 cardiac polyA+-RNA profiles of 4- to 16 week-old FVB/NJ wild type (WT) mice were generated on Illumina HiSeq 2000 instruments.

ORGANISM(S): Mus musculus

SUBMITTER: Scot Matkovich

PROVIDER: E-GEOD-55790 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulation of cardiac microRNAs by cardiac microRNAs.

Matkovich Scot J SJ Hu Yuanxin Y Dorn Gerald W GW

Circulation research 20130426 1

<h4>Rationale</h4>MicroRNAs modestly suppress their direct mRNA targets, and these direct effects are amplified by modulation of gene transcription pathways. Consequently, indirect mRNA modulatory effects of microRNAs to increase or decrease mRNAs greatly outnumber direct target suppressions. Because microRNAs are products of transcription, the potential exists for microRNAs that regulate transcription to regulate other microRNAs.<h4>Objective</h4>Determine whether cardiac-expressed microRNAs re ...[more]

PMID: 23625950

Publication: 1/2

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