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Next Generation Sequencing of Wild-Type FVB/NJ Mouse Cardiac Polyadenylated RNA After 1 Week Pressure Overload

ABSTRACT: Purpose: Next-generation sequencing (NGS) provides for quantitation of RNA abundances and comparison of RNA abundances within tissues and cells in a manner not possible with previous microarray technologies. 5 female mice were subjected to a sham operation, and 5 female mice were subjected to transverse aortic constriction (TAC). After 1 week, hearts were harvested and polyadenylated RNAs were profiled. Analyzed data have been published in Hu et al., Proc Natl Acad Sci USA. 2012;109(48):19864-9, PMID: 23150554 10 cardiac polyA+-RNA profiles of 9 week-old FVB/NJ wild type (WT) mice (5 female sham, 5 female TAC) were generated on Illumina HiSeq 2000 instruments.

ORGANISM(S): Mus musculus

SUBMITTER: Scot Matkovich

PROVIDER: E-GEOD-56890 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Epitranscriptional orchestration of genetic reprogramming is an emergent property of stress-regulated cardiac microRNAs.

Hu Yuanxin Y Matkovich Scot J SJ Hecker Peter A PA Zhang Yan Y Edwards John R JR Dorn Gerald W GW

Proceedings of the National Academy of Sciences of the United States of America 20121112 48

Cardiac stress responses are driven by an evolutionarily conserved gene expression program comprising dozens of microRNAs and hundreds of mRNAs. Functionalities of different individual microRNAs are being studied, but the overall purpose of interactions between stress-regulated microRNAs and mRNAs and potentially distinct roles for microRNA-mediated epigenetic and conventional transcriptional genetic reprogramming of the stressed heart are unknown. Here we used deep sequencing to interrogate mic ...[more]

PMID: 23150554

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Next Generation Sequencing of Wild-Type FVB/NJ Mouse Cardiac Polyadenylated RNA

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