Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of rat blood after exposure to acetaminophen (APAP) - dose response


ABSTRACT: Gene expression training data set from rat blood samples exposed to either 150, 1500 or 2500 mg/kg of APAP for 6, 12 or 24 hours. Experiment Overall Design: Male F344/N rats, 10-12 weeks old, were exposed to 0 (vehicle only), 150, 1500 or 2500 mg/kg APAP in 0.5% ethyl cellulose by oral gavage in two doses to increase absorption. The animals were sacrificed after 6, 12 or 24 hours. Equal amounts of RNA from the blood from each of four vehicle-only treated animals at the 6 and 12 hour time point and from each of six vehicle-only treated animals at the 24 hour time point, were pooled for control gene expression. The pooled controls were compared with individual treated animals at each dose and time period. The samples were hybridized in duplicate for each individual rat for a total of 8 (exception due to insufficient RNA quality: 150 mg/kg APAP and 1500 mg/kg APAP at 12 hour time point only 3 animals) microarray chips per dose and time period. Experiment Overall Design: Experiments were performed according to the guidelines established in the NIH Guide for the Care and Use of Laboratory Animals and an approved Animal Study Protocol was on file prior to initiation of the study.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Pierre Robert Bushel 

PROVIDER: E-GEOD-5593 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Blood gene expression signatures predict exposure levels.

Bushel P R PR   Heinloth A N AN   Li J J   Huang L L   Chou J W JW   Boorman G A GA   Malarkey D E DE   Houle C D CD   Ward S M SM   Wilson R E RE   Fannin R D RD   Russo M W MW   Watkins P B PB   Tennant R W RW   Paules R S RS  

Proceedings of the National Academy of Sciences of the United States of America 20071102 46


To respond to potential adverse exposures properly, health care providers need accurate indicators of exposure levels. The indicators are particularly important in the case of acetaminophen (APAP) intoxication, the leading cause of liver failure in the U.S. We hypothesized that gene expression patterns derived from blood cells would provide useful indicators of acute exposure levels. To test this hypothesis, we used a blood gene expression data set from rats exposed to APAP to train classifiers  ...[more]

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