Transcription profiling of male F344/N rats treated with APAP (time series)

ABSTRACT: Gene expression test data set from rat liver samples exposed to either 150, 1500 or 2000 mg/kg of APAP for 3, 6 or 24 hours. The Supplementary file (appended below) contains the mapping for the decoding of blinded samples. Experiment Overall Design: Male F344/N rats, 10-12 weeks old, were exposed to 0 (vehicle only), 150, 1500 or 2000 mg/kg APAP in 0.5% ethyl cellulose by oral gavage in two doses to increase absorption. The animals were sacrificed after 3, 6, or 24 hours. At the time of sacrifice, liver sections from the left lobe were isolated, cubed, flash frozen and pulverized. Total hepatic RNA was isolated from individual rat livers. Equal amounts of RNA from the liver from each of six vehicle-only treated control animals were pooled at each time point and compared to individual control and treated animals at each dose and time period. The samples were hybridized in duplicate for each individual rat for a total of 12 (test set) microarray chips per dose and time period. Experiment Overall Design: Experiments were performed according to the guidelines established in the NIH Guide for the Care and Use of Laboratory Animals and an approved Animal Study Protocol was on file prior to initiation of the study.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Pierre Robert Bushel 

PROVIDER: E-GEOD-5595 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress