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Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from Saccharomyces cerevisiae strains deleted for the THSC/TREX-2 subunits Thp1, Sac3 and Sus1

ABSTRACT: Transcription is a major obstacle for replication fork progression and a cause of genome instability. Such instability increases in mutants with a suboptimal assembly of the nascent messenger ribonucleo-protein particle (mRNP), as THO/TREX and the NPC-associated THSC/TREX-2 complex. We used microarrays to analyze the global impact of THSC/TREX-2 in gene expression and found that Thp1 and Sac3 depletion has a functional impact in highly-expressed, long and G+C-rich genes regardless of their function S. cerevisiae strains were grown in YPD liquid culture, total RNA was isolated and hybridized on Affymetrix microarrays

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Andres Aguilera

PROVIDER: E-GEOD-56702 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A genome-wide function of THSC/TREX-2 at active genes prevents transcription-replication collisions.

Santos-Pereira José M JM García-Rubio María L ML González-Aguilera Cristina C Luna Rosa R Aguilera Andrés A

Nucleic acids research 20141007 19

The THSC/TREX-2 complex of Saccharomyces cerevisiae mediates the anchoring of transcribed genes to the nuclear pore, linking transcription elongation with mRNA export and genome stability, as shown for specific reporters. However, it is still unknown whether the function of TREX-2 is global and the reason for its relevant role in genome integrity. Here, by studying two TREX-2 representative subunits, Thp1 and Sac3, we show that TREX-2 has a genome-wide role in gene expression. Both proteins show ...[more]

PMID: 25294824

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