Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of human lobular and ductal invasive carcinomas vs normal breast tissue from the same patient to identify gene expression profiles of ductal and lobular carcinomas in relation to normal ductal and lobular cells


ABSTRACT: The aim of our study was to identify gene expression profiles of ductal and lobular carcinomas in relation to normal ductal and lobular cells. We examined ten mastectomy specimens from postmenopausal breast cancer patients. Ductal and lobular tumor and normal cells were microdissected from cryosections. Fifty nanograms of total RNA were amplified and labeled by PCR and in vitro transcription. GCOS pairwise comparison algorithm and rank products have identified multiple genes that are differentially expressed in comparisons between ductal and lobular tumor and normal cell types. The results suggest that these genes are involved in epithelial-mesenchymal transition, TGFbeta and Wnt signaling. These changes are present in both tumor types but appear to be more prominent in lobular carcinomas. Ten surgical specimens obtained by mastectomy from postmenopausal patients with invasive ductal (IDC) and lobular breast (ILC) carcinomas were investigated. Of these, 5 were IDCs and 5 were ILCs. Tumor and normal tissues from the same mammary gland were identified by an experienced pathologist, snap-frozen in liquid nitrogen and stored at -80ºC for further analysis. Microdissection, RNA isolation, amplification, labeling and microarray analysis are described in sample definitions. Samples from particular cell types (normal ductal, normal lobular, tumor ductal, tumor lobular - 10, 10, 5, 5 samples, respectively) were considered as biological replicates and were compared in between.

ORGANISM(S): Homo sapiens

SUBMITTER: Jan Bouchal 

PROVIDER: E-GEOD-5764 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Novel markers for differentiation of lobular and ductal invasive breast carcinomas by laser microdissection and microarray analysis.

Turashvili Gulisa G   Bouchal Jan J   Baumforth Karl K   Wei Wenbin W   Dziechciarkova Marta M   Ehrmann Jiri J   Klein Jiri J   Fridman Eduard E   Skarda Jozef J   Srovnal Josef J   Hajduch Marian M   Murray Paul P   Kolar Zdenek Z  

BMC cancer 20070327


<h4>Background</h4>Invasive ductal and lobular carcinomas (IDC and ILC) are the most common histological types of breast cancer. Clinical follow-up data and metastatic patterns suggest that the development and progression of these tumors are different. The aim of our study was to identify gene expression profiles of IDC and ILC in relation to normal breast epithelial cells.<h4>Methods</h4>We examined 30 samples (normal ductal and lobular cells from 10 patients, IDC cells from 5 patients, ILC cel  ...[more]

Similar Datasets

2007-03-16 | GSE5764 | GEO
2007-03-27 | E-SMDB-3962 | biostudies-arrayexpress
2022-05-26 | GSE203589 | GEO
2010-08-20 | E-NCMF-3 | biostudies-arrayexpress
2021-12-31 | GSE148156 | GEO
2007-03-09 | E-SMDB-3963 | biostudies-arrayexpress
| S-EPMC5873281 | biostudies-literature
| phs001006 | dbGaP
2008-11-01 | GSE12708 | GEO
| PRJNA97101 | ENA