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Gene expression changes after loss of Hoxa9 in transformed HSCs [HOXA9 RNA-seq]

ABSTRACT: Characterization of gene expression changes 72 hours after withdrawal of tamoxifen in murine hematopoietic progenitors transformed by Hoxa9-ER/Meis1 using RNAseq. In the presence of tamoxifen (4OHT), Hoxa9-ER localizes to the nucleus of cells allowing for transformation, while withdrawal of 4OHT (culture in EtOH) leads to loss of nuclear Hoxa9-ER. Loss of Hoxa9-ER leads to a decrease in cellular proliferation and differentiation along the myeloid lineage. Examination of gene expression by RNAseq in two conditions in biological replicates.

ORGANISM(S): Mus musculus

SUBMITTER: Jay Hess

PROVIDER: E-GEOD-58359 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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C/EBPα is an essential collaborator in Hoxa9/Meis1-mediated leukemogenesis.

Collins Cailin C Wang Jingya J Miao Hongzhi H Bronstein Joel J Nawer Humaira H Xu Tao T Figueroa Maria M Muntean Andrew G AG Hess Jay L JL

Proceedings of the National Academy of Sciences of the United States of America 20140623 27

Homeobox A9 (HOXA9) is a homeodomain-containing transcription factor that plays a key role in hematopoietic stem cell expansion and is commonly deregulated in human acute leukemias. A variety of upstream genetic alterations in acute myeloid leukemia (AML) lead to overexpression of HOXA9, almost always in association with overexpression of its cofactor meis homeobox 1 (MEIS1) . A wide range of data suggests that HOXA9 and MEIS1 play a synergistic causative role in AML, although the molecular mech ...[more]

PMID: 24958854

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