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3' RNA-seq of fly expressing cabut RNAi after exposure to different levels of sucrose

ABSTRACT: Control (+/cbtE1-UAS-cbt RNAi) or cabut RNAi flies (Tim-gal4, UAS-cbt RNAi) were starved for 16 hours and then exposed to food containing different concentrations of sucrose: 0, 25, 50 and 100 % for 18 hours. Fly heads were collected, RNA was extracted and RNA-seq libraries were prepared as previously described (Engreitz et al., 2013) For each sucrose concentration, two samples of cabut RNAi flies and one sample of control flies were sequenced.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sebastian Kadener

PROVIDER: E-GEOD-58854 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing.

Bartok Osnat O Teesalu Mari M Ashwall-Fluss Reut R Pandey Varun V Hanan Mor M Rovenko Bohdana M BM Poukkula Minna M Havula Essi E Moussaieff Arieh A Vodala Sadanand S Nahmias Yaakov Y Kadener Sebastian S Hietakangas Ville V

The EMBO journal 20150427 11

Nutrient sensing pathways adjust metabolism and physiological functions in response to food intake. For example, sugar feeding promotes lipogenesis by activating glycolytic and lipogenic genes through the Mondo/ChREBP-Mlx transcription factor complex. Concomitantly, other metabolic routes are inhibited, but the mechanisms of transcriptional repression upon sugar sensing have remained elusive. Here, we characterize cabut (cbt), a transcription factor responsible for the repressive branch of the s ...[more]

PMID: 25916830

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3' RNA-seq of flies over-expressing cabut protein or RNAi against cabut mRNA

Project description:CbtOE (Tim-gal4; UAS-cbtFLAG), Tim-gal4 (control for CbtOE), cbtRNAi (Tim-gal4-UAS-Dcr2-UAS-cbtIR-cbtE1) and Tim-gal4;UAS-Dcr2 (control for CbtRNAi) flies. Flies were entrained in LD (light: dark) condition for 3-4 days and harvested at six time points: ZT3, ZT7, ZT11, ZT15, ZT19, ZT23 Fly heads were collected, RNA was extracted and RNA-seq libraries were prepared as previously described (Engreitz et al., 2013) Three samples of cbtRNAi and three samples of their controls. Two samples of cbtOE with two samples of their controls.

2015-05-08 | E-GEOD-67590 | biostudies-arrayexpress

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Expression data from 0.15M and 0.7M-fed wild-type and ChREBP mutant, third instar Drosophila larval fat bodies (FBs)

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