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Genome wide expession analysis of effect of Batf2 knock down in bone marrow derived macrophage cells stimulated with IFNg

ABSTRACT: Bmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by transfection with Batf2 ShRNA. Media was replanished in every two days and on 10th day cells were stimulated with IFNg for 4 hrs. Total RNA was obtain after 4 hrs of stimulation. Total RNA was colloected from control ShRNA transfected Bmdm cells and Batf2 shRNA transfected Bmdm cells stimulated with/with out IFNg

ORGANISM(S): Mus musculus

SUBMITTER: Harukazu Suzuki

PROVIDER: E-GEOD-59209 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome wide expession analysis of effect of Batf2 knock down in bone marrow derived macrophage cells stimulated with IFNg

Project description:Bmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by transfection with Batf2 ShRNA. Media was replanished in every two days and on 10th day cells were stimulated with IFNg for 4 hrs. Total RNA was obtain after 4 hrs of stimulation.

2015-04-30 | GSE59209 | GEO

Genome wide expession analysis of mouse bone marrow derive macrophage (Bmdm) cell stimulated with IFNg

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2015-02-26 | E-GEOD-56736 | biostudies-arrayexpress

IFNg+ vs IFNg- Treg

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Project description:We studied the effect of knowking down SUZ12 +/- knowckdown of BRM on the responsivness of IFNg stimulated genes. Cells were transfected with siSZU12+/-siBRM or control siRNA+/-siBRM. Cells were then left untreated or exposed to IFNg for 6 hours. Total RNA was collected and analyzed with Illumina arrays. SW13 cells were transfected with siSUZ12+/-siBRM or control siRNA+/-siBRM. Cells were left untreated or exposed to IFNg for 6 hours.

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