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Genome wide expession analysis of effect of Batf2 knock down in bone marrow derived macrophage cells stimulated with IFNg

ABSTRACT: Bmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by transfection with Batf2 ShRNA. Media was replanished in every two days and on 10th day cells were stimulated with IFNg for 4 hrs. Total RNA was obtain after 4 hrs of stimulation.

ORGANISM(S): Mus musculus

PROVIDER: GSE59209 | GEO | 2015/04/30

SECONDARY ACCESSION(S): PRJNA254729

REPOSITORIES: GEO

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Genome wide expession analysis of effect of Batf2 knock down in bone marrow derived macrophage cells stimulated with IFNg

Project description:Bmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by transfection with Batf2 ShRNA. Media was replanished in every two days and on 10th day cells were stimulated with IFNg for 4 hrs. Total RNA was obtain after 4 hrs of stimulation. Total RNA was colloected from control ShRNA transfected Bmdm cells and Batf2 shRNA transfected Bmdm cells stimulated with/with out IFNg

2015-04-30 | E-GEOD-59209 | biostudies-arrayexpress

Genome wide expession analysis of mouse bone marrow derive macrophage (Bmdm) cell stimulated with IFNg

Project description:Bmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by cytokine stimulation total RNA was harvested at 2, 4, 6, 24 hrs Total RNA was collected from bmdm cells stimulated with IFNg at different time point

2015-04-30 | E-GEOD-59207 | biostudies-arrayexpress

Genome wide expession analysis of mouse bone marrow derive macrophage (Bmdm) cell stimulated with IFNg

Project description:Bmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by cytokine stimulation total RNA was harvested at 2, 4, 6, 24 hrs

2015-04-30 | GSE59207 | GEO

Genome wide expession analysis of mouse bone marrow derive macrophage (Bmdm) cell stimulated with cytokine and infected with mycobacterium tuberculosis

2015-02-26 | GSE56736 | GEO

Genome wide expession analysis of mouse bone marrow derive macrophage (Bmdm) cell stimulated with cytokine and infected with mycobacterium tuberculosis

Project description:Bmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by cytokine stimulation after 24 hrs cells were infected with mycobacterium tuberculosis to identify the host factors involved in infection. Total RNA was colloected from bmdm cells stimulated with cytokine and /or infected with mycobacterium tuberculosis in a time course experiments from 28hrs to 72 hrs.

2015-02-26 | E-GEOD-56736 | biostudies-arrayexpress

Effect of Batf2 deficiency on gene epxression in murine alveolar macrophages treated ex vivo with IFNg and K. pneumoniae supernatant

Project description:Basic leucine zipper transcription factor ATF-like 2 (BATF2) is a transcription factor that is emerging as an important regulator of the innate immune system. We previously identified BATF2 among the top upregulated genes in human alveolar macrophages treated with LPS, but the signaling pathways that induce BATF2 expression in response to Gram-negative stimuli are incompletely understood. In addition, the role of BATF2 in the host response to pulmonary infection with a Gram-negative pathogen like Klebsiella pneumoniae (Kp) is not known. We show that induction of Batf2 gene expression in macrophages in response to Kp in vitro requires TRIF and type I interferon (IFN) signaling, but not MyD88 signaling. Analysis of the impact of BATF2-deficiency on macrophage effector functions in vitro showed that BATF2 does not directly impact macrophage phagocytic uptake and intracellular killing of Kp. However, BATF2 markedly enhanced macrophage pro-inflammatory gene expression and Kp-induced cytokine responses. In vivo, we observed that Batf2 gene expression was elevated in the lung tissue of wild type (WT) mice 24 h after pulmonary Kp infection, and Kp-infected BATF2-deficient (Batf2-/-) mice displayed a modest but significant increase in bacterial burden in the lung, spleen and liver compared to WT mice. WT and Batf2-/- mice showed similar recruitment of leukocytes following infection, but in line with our in vitro observations, pro-inflammatory cytokine levels in the alveolar space were reduced in Batf2-/- mice. Altogether, these results suggest that BATF2 enhances pro-inflammatory cytokine responses in macrophages in response to Kp, and that as such, BATF2 contributes to the host defense against pulmonary Kp infection.

2023-08-31 | GSE235114 | GEO

Genome-wide identification of BATF2 binding sites in HeLa-BATF2 cells

Project description:Basic leucine zipper transcription factors (BATFs) play profound roles in the regulatory networks of gene expression in tumor cells. BATF2, also known as SARI (suppressor of AP-1, regulated by interferon), was able to suppress both growth and migration of various cancers. In searching for BATF2/SARI-specific regulatory DNA elements, we first established a stable BATF2 over-expression HeLa cell line, then employed ChIP-Sequencing in order to identify the responsive DNA motifs for BATF2/SARI.

2015-03-26 | GSE67258 | GEO

Differential gene expression in IFNg+ and IFNg- IL-15 primed NK cells after anti-NK1.1 stimulation [IFNgReporter_RNA-seq]

Project description:We isolated splenic NK cells and cultured them for 3 days in 100 ng/ml IL-15. Next, we stimulated the cells with plate-bound anti-NK1.1 for 6 hours and sorted YFP+ (IFNg+) and YFP- (IFNg-) populations. We then performed gene expression profiling analysis using data obtained from RNA-seq from unstimulated, YFP+ or YFP- NK cells.

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A comprehensive analysis of gene expression patterns in wild-type and Batf2-/- bone marrow-derived macrophages (BMMφ)

Project description:We previously identified TLR-independent expression of 4933430F08Rik, encoding Batf2, in T. cruzi-infected bone marrow-derived dendritic cells (BMDCs) (Kayama et al., 2009). To determine the functions of Batf2 in innate immune responses, we performed a comprehensive gene expression analysis in wild-type and Batf2-/- bone marrow-derived macrophages (BMMφ). RNA-seq analysis showed that 98 genes are upregulated in Batf2-/- BMMφ stimulated with LPS following IFN-γ treatment, when compared with that in wild-type cells. Among these genes, we focused on Il23a, encoding IL-23p19, because IL-23 is able to promote expression of Il17a in Th17 cells.

2017-03-27 | GSE81724 | GEO

IFNg+ vs IFNg- Treg

Project description:Gene expression studies comparing IFNg+ Tregs versus IFNg- Tregs from human peripheral blood Ex vivo sorted Tregs (CD25highCD127neg) were stimulated for 4 hours and IFNg-secreting cells were detected by a IFNg-capture kit. The samples were resorted based on IFNg expression.

2016-07-01 | E-GEOD-71274 | biostudies-arrayexpress

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