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RNA-Seq analysis to profile the transcriptome of Bone Marrow Dendritic Cells exposed to different antigen delivery systems

ABSTRACT: Here we report the analysis of the transcriptome of bone marrow-derived dendritic cells exposed to the different antigen delivery systems to dissect the molecular mechanisms that orchestrate the immune response upon vaccination. RNA-Sequencing revealed that exposure of dendritic cells to diffent antigen display vehicules causes the deregulation of molecules involved in the immune response, at a different extent, depending on the carrier. In vaccinology, patterns of gene expression induced by priming antigen presenting cells (APCs), could be used to predict immunogenicity and the type of T-helper cell polarization, and/or to helpin selecting an appropriate adjuvant to administer in the vaccine formulation. Bone marrow precursor at day 7 of GM-CSF culture were exposed or not to LPS deprived bacteriophage, bacteriophage modified to be targeted to DC or to the E2 protein scaffold

ORGANISM(S): Mus musculus

SUBMITTER: Piergiuseppe De Berardinis

PROVIDER: E-GEOD-60231 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-Seq analysis to profile the transcriptome of Bone Marrow Dendritic Cells exposed to different antigen delivery systems

Project description:Here we report the analysis of the transcriptome of bone marrow-derived dendritic cells exposed to the different antigen delivery systems to dissect the molecular mechanisms that orchestrate the immune response upon vaccination. RNA-Sequencing revealed that exposure of dendritic cells to diffent antigen display vehicules causes the deregulation of molecules involved in the immune response, at a different extent, depending on the carrier. In vaccinology, patterns of gene expression induced by priming antigen presenting cells (APCs), could be used to predict immunogenicity and the type of T-helper cell polarization, and/or to helpin selecting an appropriate adjuvant to administer in the vaccine formulation.

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Time course of the mRNA expression after phage ϕYS40 infection in crp deletion mutant of Thermus thermophilus HB8.

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| PRJNA87663 | ENA

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